Efficiency of Adipose-Derived versus Bone Marrow-Derived Stem Cells in Modulation of Histopathological Changes and CD31 Immunoexpression during Wound Healing in Rats
Received Date: Apr 04, 2018 / Accepted Date: Apr 17, 2018 / Published Date: Apr 25, 2018
Objective: To evaluate therapeutic effect of intradermal injection of bone marrow-derived mesenchymal (BMMSCs) versus adipose-derived (ASCs) stem cells on surgical skin wound healing.
Methods: Forty albino rats were used and classified into four groups: I (Control), II (skin wound), III (skin wound with BM-MSCs treatment) and IV (skin wound with ASCs treatment). The rats were sacrificed on 7th and 14th days from the day of wound induction and skin specimens were processed to be examined by light microscope.
Results: Examination of skin sections of groups II, III and IV after 7 days revealed discontinuity of epidermis, granulation tissue formation and cellular infiltration which were more extensive in group II. After 14 days, these findings persisted in group II, partially subsided in group III and completely subsided in group IV. Collagen fibers were increased with change in their orientation as the healing progress. The CD31 immunoexpression in endothelial cells lining dermal blood vessels was increased in group IV after 7 days with significant reduction in the same group after 14 days when compared with group II and group III.
Conclusion: Wound healing was more advanced with ASCs treatment and that could suggest ASCs as a promising therapy for wound healing without complications and further investigations are recommended to enhance the efficacy of ASCs as a therapy for tissue repair.
Keywords: Adipose stem cells; CD31; Wound; Microscope
Citation: Hashem HE , Mobasher MOI, Mohamed MZ, Alkhodary AAM (2018) Efficiency of Adipose-Derived versus Bone Marrow-Derived Stem Cells in Modulation of Histopathological Changes and CD31 Immunoexpression during Wound Healing in Rats. J Biochem Cell Biol 1: 106.
Copyright: © 2018 Hashem HE, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.