Elimination of Antibiotic Multi-Resistant Salmonella Typhimurium from
Swine Wastewater by Microalgae-Induced Antibacterial Mechanisms
Melissa Paola1, Jean Michel Prandinib1, Jalusa Deon Kich2 and Márcio Luís Busi da Silva3*
1Biotechnology and Sciences Program, West University of Santa Catarina, Videira, SC 89560-000, Brazil
2Department of Chemical Engineering, Federal University of Santa Catarina, Florianópolis, SC 88040-900, Brazil
3EMBRAPA Swine and Poultry, Concórdia, Brazil
- *Corresponding Author:
- Márcio Luís Busi da Silva
EMBRAPA Swine and Poultry
E-mail: [email protected]
Received date: December 13, 2016; Accepted date: January 06, 2017; Published date: January 09, 2017
Citation: Paola M, Prandinib JM, Kich JD, Silva MLBd (2017) Elimination of Antibiotic Multi-Resistant Salmonella typhimurium from Swine Waste Water by Microalgae-Induced Antibacterial Mechanisms. J Bioremediat Biodegrad 8:379. doi: 10.4172/2155-6199.1000379
Copyright: © 2017 Paola M, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
The effect of microalgae-based swine wastewater treatment on the removal of antibiotic multi-resistant Salmonella enterica serovar Typhimurium was investigated. Photobioreactors (PBRs) containing diluted swine digestate with and without microalgae Scenedesmus spp. were inoculated with S. Typhimurium (108 Colony Forming Units per milliliters - CFU mL-1). Viable cells of S. Typhimurium were quantified over time by plate counts and qPCR amplification of the Salmonella invasion gene activator, hilA. In the absence of microalgae, S. Typhimurium concentrations increased 1.5 log cells mL-1 in 96 h. In the presence of microalgae, S. Typhimurium was completely eradicated within 48 h. In the PBRs with controlled pH (6.8 ± 0.8), concentration of S. Typhimurium remained constant (2.8 ± 0.2 log CFU mL-1) throughout 96 h. Thus, natural increase in pH>10 due to photosynthesis was detrimental to the antibiotic multiresistant bacteria survival. Phycoremediation holds promises as an alternative for wastewater treatment process for the elimination of the serious public health threatening antibiotic multi-resistant bacteria, thus effectively avoiding Salmonellosis outbreaks arising from animal farming activities.