Bacillus cereus Mediated ε-Caprolactam Degradation: An Initiative for Waste Water Treatment of Nylon-6 Production Plant.
|Mehta SK, Panchal PA, Butala BN and Sane SA*|
|Department of Biotechnology, Research and Development Centre, Gujarat State Fertilizers and Chemicals Limited, Vadodara-391750, India|
|Corresponding Author :||Sane SA
Department of Biotechnology
Research and Development centre
GSFC Ltd., Vadodara 391750, India
Tel: +91 9712986358
Fax: +91 0265 2240966
|Received May 14, 2014; Accepted June 05, 2014; Published June 09, 2014|
|Citation: Mehta SK, Panchal PA, Butala BN, Sane SA (2014) Bacillus cereus Mediated ε-Caprolactam Degradation: An Initiative for Waste Water Treatment of Nylon-6 Production Plant. J Bioremed Biodeg 5:230. doi:10.4172/2155-6199.1000230|
|Copyright: © 2014 Mehta SK, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Present study focuses on isolation, characterization and application of a novel bacterial strain for biological treatment of waste water from Nylon 6 production plant of Gujarat State Fertilizers and Chemicals (GSFC), Vadodara. This microbe was isolated by applying basal synthetic medium containing ε-caprolactam (precursor of Nylon-6 polymer) as a sole source of carbon and nitrogen. It was identified as Bacillus cereus strain YH-10 on the basis of primary characterization followed by 16s ribosomal RNA gene analysis. This strain has very high tolerance for ε-caprolactam (3.5%) compared to other reported ε-caprolactam -degrading microbes. It degrades 91% and 83.3% ε-caprolactam of synthetic media and Nylon-6 Waste water, respectively in 96 hrs. ε-caprolactam degradation was calculated by analyzing unutilized ε-caprolactam concentration in the samples using liquid chromatography-mass spectroscopy (LC-MS/MS) and chemical oxygen demand (COD) analysis. Based on these observations, biological treatment of waste water from Nylon-6 plant is envisaged utilizing minimum resources.