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Hindi Research Article
Enzyme-Mediated Bioremediation of Organophosphates Using Stable Yeast Biocatalysts
| Randhir S. Makkar1, Augustine A. DiNovo1, Caroline Westwater2 and David A. Schofield1* | |
| 1Guild Associates, Inc., Charleston, South Carolina, 29407, USA | |
| 2Department of Craniofacial Biology, Medical University of South Carolina, South Carolina, 29425, USA | |
| Corresponding Author : | David A Schofield Guild Associates Inc. 1313B Ashley River Road Charleston, SC 29407, USA Tel: +(843) 573 0095 Fax: +(843) 573 0707 E-mail: dschofield@guildassociates.com |
| Received: February 08, 2013; Accepted: February 21, 2013; Published: February 23, 2013 | |
| Citation: Makkar RS, DiNovo AA, Westwater C, Schofield DA (2013) Enzyme- Mediated Bioremediation of Organophosphates Using Stable Yeast Biocatalysts. J Bioremed Biodeg 4:182. doi:10.4172/2155-6199.1000182 | |
| Copyright: © 2013 Makkar RS, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Organophosphates are extremely toxic compounds, which pose a threat to the environment and public due to
their widespread use as common pesticides or due to their deliberate release as chemical weapons. The bacterial enzyme organophosphorus hydrolase (OPH, EC. 3.1.8.1) can hydrolyze, and thereby detoxify a broad range of organophosphate nerve agents. This enzyme therefore offers the opportunity for the development of naturally occurring newer bioremediation strategies. The aim of this research was to generate a stable yeast biocatalyst that was capable of hydrolyzing the poorly hydrolyzed P-S class of organophosphates. The genes encoding the wild-type OPH, or the enhanced variant enzyme S308L-OPH, were integrated into the ribosomal operon of the Saccharomyces cerevisiae genome to create a stable yeast biocatalyst.
