Estimation of Pharmacokinetics of Propofol in Indian Pateints by HPLC MethodA Puri1*, B Mehdi1, N B Panda1 and G D Puri S Dhawan2
- *Corresponding Author:
- Dr. Puri
Post Graduate Institute of Medical Education
and Research# India
E-mail: [email protected]
Received date: March 26, 2011; Accepted date: May 16, 2011; Published date: May 17, 2011
Citation: Puri A, Mehdi B, Panda NB, Dhawan GDPS(2011) Estimation of Pharmacokinetics of Propofol in Indian Pateints by HPLC Method. J Anal Bioanal Tech 2:120. doi: 10.4172/2155-9872.1000120
Copyright: © 2011 Puri A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A quick and sensitive reversed phase high performance liquid chromatography (HPLC) method has been developed in Indian surgical patients to determine the concentration of Propofol In human plasma. Propofol can be isolated from human plasma by adding 1ml precipitating solution which consists of acetonitrile and perchloric acid (67:33) mixture, which also contains dibutylpthalate (1mg/ml) as an internal standard. The sample is mixed for two minute on a vortexer. The plasma substance precipitated by acetonitrile and perchloric acid are further separated by centrifugation. The supernatant is directly injected into the HPLC system with the help of autosampler.
The analysis was carried out using column 250 × 4.6 mm column packed with10-μm Spherisorb reversed phase octadecyl silane particles (C 18 ). The 500ml of mobile phase (67:33:0.04) consisted of 335ml of acetonitrile and 165ml of distilled water and 200μl of acetic acid maintaining the pH 4.0.The flow rate of the mobile phase was 1.5ml/ min. propofol was monitored by a UV detector at a 270nm wavelength. The limit of detection of propofol (in human plasma) was found to be 0.0001μg/ml while limit of quantification was found to be 0.001μg/ml for a 20ul injection volume.