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Evaluation of Intact Cell Matrix-Assisted Laser Desorption/Ionization Timeof-Flight Mass Spectrometry for Capillary Electrophoresis Detection of Controlled Bacterial Clumping
Pomastowski P1,2, Szultka M1,2, Kupczyk W3,2, Jackowski M3,2 and Buszewski B1,2*1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Poland
2Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Poland
3Department of Surgery, Collegium Medicum, Nicolaus Copernicus University, Poland
- *Corresponding Author:
- Buszewski B
Department of Environmental Chemistry and Bioanalytics
Faculty of Chemistry, Nicolaus Copernicus University
Gagarina 7, 87-100 Torun, Poland
Tel: +48566114308
Fax: +48566114837
E-mail: bbusz@chem.umk.pl
Received date: October 29, 2015; Accepted date: November 18, 2015; Published date: November 25, 2015
Citation: Pomastowski P, Szultka M, Kupczyk W, Jackowski M, Buszewski B (2015) Evaluation of Intact Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Capillary Electrophoresis Detection of Controlled Bacterial Clumping. J Anal Bioanal Tech S13:008. doi:10.4172/2155-9872.S13-008
Copyright: © 2015 Pomastowski P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Abstract
Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been proposed as a technique of choice for quick classification of bacterial cells and precise characterization of microorganisms. The evaluated MALDI MS procedure was confronted with automated biochemical method using the VITEK 2 Compact system employed as a reference method. Moreover, the investigation aimed at describing and optimization of the environmental conditions having an impact on reproducibility and quality of one-dimensional intact-cell MALDI TOF spectra. The work presents off-line combination of capillary electrophoresis of microbial clumping with spectrometric detection.
