alexa

GET THE APP

Evaluation of Intact Cell Matrix-Assisted Laser Desorption/Ionization Timeof-Flight Mass Spectrometry for Capillary Electrophoresis Detection of Controlled Bacterial Clumping | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Evaluation of Intact Cell Matrix-Assisted Laser Desorption/Ionization Timeof-Flight Mass Spectrometry for Capillary Electrophoresis Detection of Controlled Bacterial Clumping

Pomastowski P1,2, Szultka M1,2, Kupczyk W3,2, Jackowski M3,2 and Buszewski B1,2*

1Department of Environmental Chemistry and Bioanalytics, Faculty of Chemistry, Nicolaus Copernicus University, Poland

2Interdisciplinary Centre of Modern Technology, Nicolaus Copernicus University, Poland

3Department of Surgery, Collegium Medicum, Nicolaus Copernicus University, Poland

*Corresponding Author:
Buszewski B
Department of Environmental Chemistry and Bioanalytics
Faculty of Chemistry, Nicolaus Copernicus University
Gagarina 7, 87-100 Torun, Poland
Tel: +48566114308
Fax: +48566114837
E-mail: [email protected]

Received date: October 29, 2015; Accepted date: November 18, 2015; Published date: November 25, 2015

Citation: Pomastowski P, Szultka M, Kupczyk W, Jackowski M, Buszewski B (2015) Evaluation of Intact Cell Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry for Capillary Electrophoresis Detection of Controlled Bacterial Clumping. J Anal Bioanal Tech S13:008. doi:10.4172/2155-9872.S13-008

Copyright: © 2015 Pomastowski P, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

Matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been proposed as a technique of choice for quick classification of bacterial cells and precise characterization of microorganisms. The evaluated MALDI MS procedure was confronted with automated biochemical method using the VITEK 2 Compact system employed as a reference method. Moreover, the investigation aimed at describing and optimization of the environmental conditions having an impact on reproducibility and quality of one-dimensional intact-cell MALDI TOF spectra. The work presents off-line combination of capillary electrophoresis of microbial clumping with spectrometric detection.

Keywords

Top