alexa Exploited Application of Bacillus spp. ETL-1979 for Degradation and Decolorization of Methyl Orange, Malachite Green and Congo Red
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
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Research Article

Exploited Application of Bacillus spp. ETL-1979 for Degradation and Decolorization of Methyl Orange, Malachite Green and Congo Red

Maulin P Shah1*, Kavita A Patel1, Sunu S Nair1, A M Darji1 and Shaktisinh Maharaul2
1Industrial Waste Water Research Laboratory, Applied and Environmental Microbiology Lab, Enviro Technology Limited (CETP), Gujarat, India
2Laboratory of Environmental Bioremediation, Narmada Clean Tech Limited (FETP), Ankleshwar, Gujarat, India
Corresponding Author : Maulin P Shah
Industrial Waste Water Research Laboratory
Applied and Environmental Microbiology Lab, Enviro Technology Limited (CETP)
Plot No: 2413/2414, GIDC, Ankleshwar- 393 002, Gujarat, India
Tel: +91-90 999 65504
Fax: +91-2646-250707
E-mail: [email protected]
Received: June 15, 2013; Accepted: July 29, 2013; Published: July 31, 2013
Citation: Shah MP, Patel KA, Nair SS, Darji AM, Maharaul S (2013) Exploited Application of Bacillus spp. ETL-1979 for Degradation and Decolorization of Methyl Orange, Malachite Green and Congo Red. J Bioremed Biodeg 4:196. doi:10.4172/2155-6199.1000196
Copyright: © 2013 Shah MP, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The local industrial dye waste water sample was collected from Final Effleunt Treatment Plant (FETP) of Ankelshwar, Gujarat, India for the isolation of dye degrading bacteria. The isolate ETL-1979 was selected based on its maximum decolorization of dyes Methyl orange, Congo red and Malachite green (each 150 mg/L). Phenotypic characterization and phylogenetic analysis based on 16S rDNA sequence confirmed ETL-1979 as Bacillus sp. On physiochemical parameters optimization, Bacillus sp. ETL-1979 degraded 10.13% (0.15 mg/mL), 11.76% (0.11 mg/ mL) and 9.32% (0.8 mg/mL) of Methyl orange, Congo red and Malachite green, respectively within 2 h, whilst 100% degradation (2.97 mg/mL) at 18 h for Methyl orange, 100% (2.43 mg/mL) at 16 h for Congo red and 100% (3.18 mg/mL) at 20 h for Malachite green were recorded. The degraded products were found non-toxic in nature, while bacterial cells showed elongation and membrane rupturing under SEM taken from dye degraded media.

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