Expression Profile of Angiogenesis Related Genes in Head and Neck Squamous Cell Carcinoma
|S. Jung1*, S. Sielker1,2, N. Purcz3, C. Sproll4, A. Raem2 and J. Kleinheinz1|
|1Research Unit Vascular Biology of Oral Structures (VABOS), Department of Cranio-Maxillofacial Surgery, University Hospital Muenster, Waldeyerstraße 30, D-48149 Muenster, Germany|
|2Arrows biomedical Deutschland GmbH, Muenster, Germany|
|3Department of Cranio-Maxillofacial Surgery, University Hospital Kiel, Germany|
|4Department of Cranio-Maxillofacial Surgery, University Hospital Duesseldorf, Germany|
|Corresponding Author :||Susanne Jung MD, DMD
Research Unit Vascular Biology of Oral Structures (VABOS)
Department of Cranio-Maxillofacial Surgery
University Hospital Muenster, Waldeyerstraße 30
D-48149 Muenster, Germany
E-mail: [email protected]
|Received January 04, 2013; Accepted January 23, 2013; Published February 01, 2013|
|Citation: Jung S, Sielker S, Purcz N, Sproll C, Raem A, et al. (2013) Expression Profile of Angiogenesis Related Genes in Head and Neck Squamous Cell Carcinoma. J Biochip Tissue Chip 3:104. doi:10.4172/2153-0777.1000104|
|Copyright: © 2013 Jung S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
|Related article at
Pubmed Scholar Google
Objectives: The molecular patterns of tumorigenesis of head and neck squamous cell carcinoma are mostly unknown to date. The concept of this investigation was to expose a genetic fingerprint of angiogenesis in HNSCC with special regard to VEGF as the outstanding mediator of neo-angiogenesis.
Material and Methods: A panel of significant transcriptional alterations of VEGF related genes in 83 cancer samples compared to healthy mucosa with microarray technique was established. RT-PCR and immunohistochemistry were performed to confirm the microarray results.
Results: In four selected marker genes (VEGFA, RAC2, IL8, PLA2G10) we detected a highly significant
transcriptional alteration; three of the genes (VEGFA, RAC2, IL8) showed up-regulation in 88% to 100% of the samples, PLA2G10 down-regulation in 99% of the analyzed tissue samples. Additionally a significant transcription alteration in angio-miRNAs was detected: miR-21 and miR-31 were over-expressed in upto 87% and upto 89% respectively in the analyzed samples. MiR-20a, miR-126, miR-378, miR-17, miR-19a, miR-27b and miR let-7f were down-regulated in 83% to 95% of the specimens. A specific pattern subjected to the different tumor types could not be verified.
Conclusion: An expression profile of VEGF related genes was established and selective angio-miRNAs were identified as potential biomarkers on the basis of 83 HNSCC samples. Identification of new relevant oncogenes and insight into the process of tumor vasculogenesis with a highly sensitive method opens new vistas in cancer biology.