alexa Expression Profile of Angiogenesis Related Genes in Head and Neck Squamous Cell Carcinoma | OMICS International | Abstract
ISSN: 2153-0777

Journal of Bioengineering and Bioelectronics
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Expression Profile of Angiogenesis Related Genes in Head and Neck Squamous Cell Carcinoma

S. Jung1*, S. Sielker1,2, N. Purcz3, C. Sproll4, A. Raem2 and J. Kleinheinz1
1Research Unit Vascular Biology of Oral Structures (VABOS), Department of Cranio-Maxillofacial Surgery, University Hospital Muenster, Waldeyerstraße 30, D-48149 Muenster, Germany
2Arrows biomedical Deutschland GmbH, Muenster, Germany
3Department of Cranio-Maxillofacial Surgery, University Hospital Kiel, Germany
4Department of Cranio-Maxillofacial Surgery, University Hospital Duesseldorf, Germany
Corresponding Author : Susanne Jung MD, DMD
Research Unit Vascular Biology of Oral Structures (VABOS)
Department of Cranio-Maxillofacial Surgery
University Hospital Muenster, Waldeyerstraße 30
D-48149 Muenster, Germany
Tel: +49-251-834-7004
Fax: +49-251-834-7184
E-mail: [email protected]
Received January 04, 2013; Accepted January 23, 2013; Published February 01, 2013
Citation: Jung S, Sielker S, Purcz N, Sproll C, Raem A, et al. (2013) Expression Profile of Angiogenesis Related Genes in Head and Neck Squamous Cell Carcinoma. J Biochip Tissue Chip 3:104. doi:10.4172/2153-0777.1000104
Copyright: © 2013 Jung S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Abstract

 Objectives: The molecular patterns of tumorigenesis of head and neck squamous cell carcinoma are mostly unknown to date. The concept of this investigation was to expose a genetic fingerprint of angiogenesis in HNSCC with special regard to VEGF as the outstanding mediator of neo-angiogenesis.
Material and Methods: A panel of significant transcriptional alterations of VEGF related genes in 83 cancer samples compared to healthy mucosa with microarray technique was established. RT-PCR and immunohistochemistry were performed to confirm the microarray results.
Results: In four selected marker genes (VEGFA, RAC2, IL8, PLA2G10) we detected a highly significant
transcriptional alteration; three of the genes (VEGFA, RAC2, IL8) showed up-regulation in 88% to 100% of the samples, PLA2G10 down-regulation in 99% of the analyzed tissue samples. Additionally a significant transcription alteration in angio-miRNAs was detected: miR-21 and miR-31 were over-expressed in upto 87% and upto 89% respectively in the analyzed samples. MiR-20a, miR-126, miR-378, miR-17, miR-19a, miR-27b and miR let-7f were down-regulated in 83% to 95% of the specimens. A specific pattern subjected to the different tumor types could not be verified.
Conclusion: An expression profile of VEGF related genes was established and selective angio-miRNAs were identified as potential biomarkers on the basis of 83 HNSCC samples. Identification of new relevant oncogenes and insight into the process of tumor vasculogenesis with a highly sensitive method opens new vistas in cancer biology.

Top