Journal of Analytical & Bioanalytical Techniques
Open Access

Research Article

Harmonized Collaborative Validation of a Simultaneous and Multiple Determination Method for Nivalenol, Deoxynivalenol, T-2 Toxin, HT-2 Toxin, and Zearalenone in Wheat and Barley by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LC-MS/MS)

¹National Agriculture and Food Research Organization (NARO), National Food Research Institute (NFRI), 2-1-12 Kannon-dai, Tsukuba-shi, Ibaraki 305-8642, Japan

²Food and Agricultural Materials Inspection Center (FAMIC) Headquarters, Saitama, Japan

³Food and Agricultural Materials Inspection Center (FAMIC) Nagoya Regional Center, Aichi, Japan

⁴Food and Agricultural Materials Inspection Center (FAMIC) Kobe Regional Center, Hyogo, Japan

⁵Japan Grain Inspection Association, Tokyo Research Laboratory, Tokyo, Japan

⁶Asahi Group Holdings, Ltd., Research Laboratories for Food Safety Chemistry, Ibaraki, Japan

⁷Showa Sangyo Co., Ltd., Research & Development Center, Chiba, Japan

⁸Nisshin Seifun Group Inc., QE (quality exam.) Center, Saitama, Japan

⁹Kagome Co., Ltd., Research & Development Division Tochigi, Japan

¹⁰S&B Foods, Inc., Itabashi Spice Center, Tokyo, Japan

¹¹NH Foods Ltd., R&D Center, Ibaraki, Japan

¹²Co-op net Business Association, Quality Control Department, Saitama, Japan

¹³National Federation of Agricultural Co-operative Associations, Central Research Institute for Feed and Livestock, Ibaraki, Japan

¹⁴Food and Drug Safety Center (FDSC), Hatano Research Institute (HRI), Kanagawa, Japan

¹⁵Shinshu University, Faculty of Agriculture, Nagano, Japan

¹⁶National Agriculture and Food Research Organization (NARO), National Agricultural Research Center for Kyushu Okinawa Region, Kumamoto, Japan

*Corresponding Author:

Hiroyuki Nakagawa
National Agriculture and Food Research Organization (NARO)
National Food Research Institute (NFRI)
2-1-12 Kannon-dai, Tsukuba-shi
Ibaraki 305-8642, Japan
Tel: +81-29-838-8085
Fax:+81-29-838-7996
E-mail: hironkgw@affrc.go.jp

Received date: April 26, 2014; Accepted date: June 04, 2014; Published date: June 06, 2014

Citation: Nakagawa H, Naito S, Kitani Y, Ito Y, Aoyama Y, et al. (2014) Harmonized Collaborative Validation of a Simultaneous and Multiple Determination Method for Nivalenol, Deoxynivalenol, T-2 Toxin, HT-2 Toxin, and Zearalenone in Wheat and Barley by Liquid Chromatography Coupled to Tandem Mass Spectrometry (LCMS/ MS). J Anal Bioanal Techniques S6:002 doi: 10.4172/2155-9872.S6-002

Copyright: ©2014 Nakagawa H, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

Harmonized collaborative validation of a simultaneous and multiple determination method for nivalenol, deoxynivalenol, T-2 toxin, HT-2 toxin, and zearalenone in wheat and barley by liquid chromatography tandem mass spectrometry (LC-MS/MS) was conducted by participants from 12 laboratories. The fortified samples of wheat and barley at three different levels and one naturally contaminated wheat sample were extracted, consecutively purified through a Presep C18 (ODS) solid phase extraction column and a Bond Elut Mycotoxin multifunctional column, and were analyzed by LC-MS/MS. The employment of internal standards (verrucarol and zearalanone) was apparently effective to ensure repeatability and reproducibility with sufficient recovery of each mycotoxin. This is the first report of the harmonized collaborative validation study of a simultaneous and multiple determination method for both type A and B trichothecenes along with zearalenone by LC-MS/MS. The validated method should be practical for monitoring of the major Fusarium mycotoxins contained in wheat and barley.

Keywords