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HPLC-UV Method for the Determination of Enalapril in Bulk, Pharmaceutical Formulations and Serum | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

HPLC-UV Method for the Determination of Enalapril in Bulk, Pharmaceutical Formulations and Serum

Safila Naveed1*, Najma Sultana2 and M.Saeed Arayne2

1Jinnah University for Women, Karachi, Pakistan

2United Biotechnologies, Karachi-75290, Pakistan

*Corresponding Author:
Safila Naveed
Jinnah University for Women
Karachi, Pakistan
Tel: 00923002621917
E-mail: [email protected], [email protected]

Received date: December 05, 2011; Accepted date: January 17, 2012; Published date: January 20, 2012

Citation: Naveed S, Sultana N, Arayne MS (2012) HPLC-UV Method for the Determination of Enalapril in Bulk, Pharmaceutical Formulations and Serum. J Anal Bioanal Tech 3:130. doi: 10.4172/2155-9872.1000130

Copyright: © 2012 Naveed S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A simple reversed phase HPLC method have been successfully developed and validated for the quantitative determination of enalapril maleate (ENP) in bulk material, pharmaceutical formulation and serum. Purospher Start C 18 (250 cm x 4.6 mm, 5 μm) and Hypersil, ODS columns were used. The mobile phase, methanol-acetonitrile-water (70:30v/v pH 3.5 adjusted by phosphoric acid), was delivered at a flow rate of 1 mLmin -1 , eluent was monitored using UV detector at 215 nm. The proposed method is specific, accurate (99-102%), precise (intra-day and inter-day variation 0.07-1.25%) and linearity (R 2 >0.999) within the desired range 2.5-100 μgmL -1 concentration. The detection limit and quantification 3.9 ngmL -1 and 12 ngmL -1 respectively. The anticipated method is applicable to routine analysis of ENP in pharmaceutical formulations as well as in human serum samples.

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