alexa HPLC-UV Method for the Determination of Enalapril in Bulk, Pharmaceutical Formulations and Serum
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

HPLC-UV Method for the Determination of Enalapril in Bulk, Pharmaceutical Formulations and Serum

Safila Naveed1*, Najma Sultana2 and M.Saeed Arayne2

1Jinnah University for Women, Karachi, Pakistan

2United Biotechnologies, Karachi-75290, Pakistan

*Corresponding Author:
Safila Naveed
Jinnah University for Women
Karachi, Pakistan
Tel: 00923002621917
E-mail: [email protected], [email protected]

Received date: December 05, 2011; Accepted date: January 17, 2012; Published date: January 20, 2012

Citation: Naveed S, Sultana N, Arayne MS (2012) HPLC-UV Method for the Determination of Enalapril in Bulk, Pharmaceutical Formulations and Serum. J Anal Bioanal Tech 3:130. doi: 10.4172/2155-9872.1000130

Copyright: © 2012 Naveed S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



A simple reversed phase HPLC method have been successfully developed and validated for the quantitative determination of enalapril maleate (ENP) in bulk material, pharmaceutical formulation and serum. Purospher Start C 18 (250 cm x 4.6 mm, 5 μm) and Hypersil, ODS columns were used. The mobile phase, methanol-acetonitrile-water (70:30v/v pH 3.5 adjusted by phosphoric acid), was delivered at a flow rate of 1 mLmin -1 , eluent was monitored using UV detector at 215 nm. The proposed method is specific, accurate (99-102%), precise (intra-day and inter-day variation 0.07-1.25%) and linearity (R 2 >0.999) within the desired range 2.5-100 μgmL -1 concentration. The detection limit and quantification 3.9 ngmL -1 and 12 ngmL -1 respectively. The anticipated method is applicable to routine analysis of ENP in pharmaceutical formulations as well as in human serum samples.

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