Abstract

The present study is to find out the major phytochemical components and functional groups present in the leaves of Michelia nilagirica using FTIR and GC-MS. For the identification of the phytochemical constituents, TRACE 1300 Thermo Scientific gas chromatography and ISQ QD (Single Quadrapole) Thermo Scientific mass spectrometer equipped with TG-5MS capillary column (5% Diphenyl 95% dimethyl siloxane) (30 m x 0.25 mm x 0.25 μm) is used. The molecular weight and structure of the compounds were detected by investigation of the mass spectrum of GC-MS using the database of National Institute Standard and Technology (NIST) library data. For the exploration of functional groups, ATR- FTIR spectroscope (Shimadzu IR Prestige-21) is used. The GC-MS analysis of the leaves of M. nilagirica revealed the presence of twenty bioactive compounds with valuable biological activities. The FTIR analysis indicated the presence of amine, alkane, acetyl, alkene, alkyl fluoride, carbonyl and alkyl halides. The phytochemical profile of the M. nilagirica plant leaf extract specifies the occurrence of numerous bioactive compounds which can be further applied for pharmaceutical purposes.

Keywords: FT-IR; GC-MS; Medicinal property; Secondary metabolites; Western Ghats