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Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA) | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA)

Angel Justiz Vaillant1*, Norma McFarlane-Anderson2 , Brian Wisdom3 , Wayne Mohammed1 , Sehlule Vuma1 , Chalapathi Rao1 , Arvind Kurhade1 , Hellen Asemota2 , Shivnarine Kissoon1 and Geeta Kurhade4

1Department of Para-clinical Sciences, The University of the West Indies, Trinidad and Tobago

2Department of Basic Medical Sciences, The University of the West Indies, Jamaica

3School of Biology and Biochemistry, Medical Biology Centre, The Queen’s University of Belfast, UK

4Department of Pre-clinical Medical Sciences, The University of the West Indies, Trinidad and Tobago

*Corresponding Author:
Angel Justiz Vaillant
Department of Para-clinical Sciences
The University of the West Indies, St. Augustine
Trinidad and Tobago
Tel: +868-645-2640/9
E-mail: [email protected]

Received date: September 11, 2013; Accepted date: November 25, 2013; Published date: November 27, 2013

Citation: Vaillant AJ, McFarlane-Andersonv N, Wisdom B, Mohammed W, Vuma S, et al. (2013) Immunoglobulin-binding Bacterial Proteins (IBP) Conjugates and their Reactivity with Immunoglobulin in Enzyme-Linked Immunosorbent Assays (ELISA). J Anal Bioanal Tech 4: 175. doi: 10.4172/2155-9872.1000175

Copyright: © 2013 Vaillant AJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The aim of this study was to create universal chimeric conjugates able to react with both avian and mammalian immunoglobulins to be used as a reagent in Enzyme-linked Immunosorbent Assays (ELISAs). The periodate method was used in the conjugation process of linking horseradish peroxidase to immunoglobulin-binding bacterial proteins. ELISAs were used to prove the efficacy of the conjugates, namely newly synthesize conjugates (NSC) in the detection of immunoglobulins. All NSC bound to mammalian immunoglobulins, but failed to bind avian immunoglobulin Y (IgY), with the exception of the SpLAG-anti-IgY-HRP that was the most versatile binding to the all panel of purified immunoglobulin and sera. In conclusion, the results of these experiments show that SpLAG-anti-IgY-HRP conjugate, a novel reagent, was the most versatile product among NSC and commercially-available conjugates.

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