alexa In Vitro and In Vivo Effects of Aqueous Extract of Rosm
ISSN: 2329-8863

Advances in Crop Science and Technology
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Research Article

In Vitro and In Vivo Effects of Aqueous Extract of Rosmarinus officinalis L. (Rosemary) in The Control of Late Blight Disease of Potato Caused by Phytophthora Infestans Mont. De Bary. in Algeria

Messgo Moumene S1*, Olubunmi OF2, Laidani M1, Saddek D1, Houmani Z1 and Bouznad Z3
1Laboratory for Research on Medicinal and Aromatic plants, Science and life Faculty, University of Blida1, BP. 270, Soumaa road, 09100, Blida, Algeria
2Department of Crop Protection and Environmental Biology, University of Ibadan, Nigeria
3Laboratory of Phytopathology and Molecular Biology, National graduate school of Agronomy El Harrach, Algeria
Corresponding Author : Messgo-Moumene Saida
Laboratory for Research on Medicinal and Aromatic plants
Science and life Faculty, University of Blida1
BP. 270, Soumaa road, 09100, Blida, Algeria
Tel: +39-0332-789-7
E-mail: [email protected]
Received January 23, 2015; Accepted July 10, 2015; Published July 17, 2015
Citation: Moumene MS, Olubunmi OF, Laidani M, Saddek D, Houmani Z, et al. (2015) In Vitro and In Vivo Effects of Aqueous Extract of Rosmarinus officinalis L. (Rosemary) in The Control of Late Blight Disease of Potato Caused by Phytophthora Infestans Mont. De Bary. in Algeria. Adv Crop Sci Tech 3:177. doi:10.4172/2329-8863.1000177
Copyright: © 2015 Moumene MS, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

The fungus Phytophthora infestans is known to develop resistance against the metalaxyl (fungicide), commonly used in the control of potato mildew disease. There is therefore urgent need to explore the potentials of alternative fungicides which are potent, affordable, readily available, easy to prepare and environment friendly. The study was carried out to test the fungicidal potential of aqueous extracts of Rosmarinusofficinalis (Rosemary), in vitro and in vivo on two isolates of P. infestans collected from two potato producing Algerian areas: Bourkika (Tipaza City) and El Abbadia (Aindefla City). Various concentrations of crude extracts of Rosmarinus officinalis applied by direct contact in the following dilutions: 5%, 10% and 20% on medium with pea-agar (PPA), allowed the inhibition of mycelial growth of P. infestans isolates. The observed rates of inhibition exceeded 85% and the inhibitive minimal concentration (CMI) was 5%. Parallel structural modifications, caused by mycelial lyses, as well as the deformation or, and the digestion of the contents of sporangia affected the morphology of both strains from the lowest concentration. The sporulation and the germination were inhibited by this aqueous extract (100%). Also, the absence of resumption of mycelial growth on medium PPA and absence of the mildew symptoms on detached Spunta potato leaves confirmed the fungicidal effect of the Rosemary aqueous extract. This also translated in vivo as significant reduction of the disease was observed. Disease reduction was recorded for the preventive application modes by spraying with the crude aqueous extract (86.2%) and by watering, while for the curative mode with crude extract (81%). On the other hand, Spunta variety was more marked for preventive mode by watering (85%) and the curative one (90%) also, A2 isolate was more inhibited for the application of R. officinalis aqueous extract by curative (83%), spraying mode (86%) and watering modes. Besides, treatments made in preventive modes by spraying and watering showed a total inhibition of the sporulation (100%), exceeding 85% in Spunta variety and 96% for A1 isolate was observed in the curative mode of application. This study thus confirms the antifungal potential of aqueous extract of Rosmarinus officinalis on P. infestans isolates. It is thus recommended for use as bio-fungicide in the management of potato mildew disease.

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