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Hindi Research Article
Inflammasome Activation P2X7-Dependent in Crohns Disease
Zelante Angelo1*, Borgoni Riccardo4, Falzoni Simonetta2, D’Incà Renata3, Sturniolo Giacomo Carlo3, Cifalà Viviana1and Di Virgilio Francesco21Gastroenterology Unit, Department of Medicine, University Hospital "Sant'Anna", Ferrara, Italy
2Department of Experimental and Diagnostic Medicine, University of Ferrara, Italy
3Department of Surgical, Oncological and Gastroenterological Sciences, Padua, Italy
4Department of Statistics, University of Milano-Bicocca, Milan, Italy
- *Corresponding Author:
- Zelante Angelo
Gastroenterology Unit
Department of Medicine
University Hospital "Sant'Anna"
Ferrara, Italy
Tel: +0393470125037
E-mail: zelans@libero.it
Received date: June 30, 2015 Accepted date: July 16, 2015 Published: July 25, 2015
Citation:Angelo Z, Riccardo B, Simonetta F, Incà Renata D, Carlo SG, et al. (2015) Inflammasome Activation P2X7-Dependent in Crohn’s Disease. J Gastrointest Dig Syst 5: 316. doi:10.4172/2161-069X.1000316
Copyright: ©2015 Angelo Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License; which permits unrestricted use; distribution; and reproduction in any medium; provided the original author and source are credited
Abstract
The Inflammasome represents an intracellular multiprotein complex belonging to the innate immune system that identifies molecular damage. The activation of the Inflammasome triggers the maturation and secretion of cytokines IL-1β, IL-18, IL-33 which activate on his part inflammatory processes. The most important Inflammasome is NALP3 (NOD-like family) with his components P2X7, NALP3, the adapter ASC and caspase-1. NALP3 and NOD2 polymorphisms are associated with development of Crohn's disease (CD). This study analyzed the expression and function of the NALP3-inflammasome by stimulating in vitro PBMCs of CD patients. We enrolled 62 CD patients: 36 female (58.1%) and 26 male (41.9%) with a mean age of 53.7 years; the control group included 58 subjects (38 female; 20 male; mean age 46 years). The patients have been analyzed with regard to duration, location and disease activity, smoking habits, comorbidities, type of therapy, previous surgery, familiarity for IBD and CRP values. We isolated PBMCs to extract RNA for rt-PCR and proteins for Western Blotting.
