alexa Influence of Culture Medium on Production of Nitric Oxi

Journal of Cytokine Biology
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Influence of Culture Medium on Production of Nitric Oxide and Expression of Inducible Nitric Oxide Synthase by Activated Macrophages In Vitro

Fumio Amano*

Laboratory of Biodefense & Regulation, Osaka University of Pharmaceutical Sciences, Takatsuki, Osaka 569-1054, Japan

*Corresponding Author:
Fumio Amano
Laboratory of Biodefense & Regulation
Osaka University of Pharmaceutical Sciences
Takatsuki, Osaka 569-1054, Japan
Tel: 81359271020
Fax: 81359521600
E-mail: [email protected]

Received Date: May 02, 2016; Accepted Date: June 14, 2016; Published Date: June 19, 2016

Citation: Amano F (2016) Influence of Culture Medium on Production of Nitric Oxide and Expression of Inducible Nitric Oxide Synthase by Activated Macrophages In Vitro. J Cytokine Biol 1:108. doi: 10.4172/jcb.1000108

Copyright: © 2016 Amano F. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited



Activated macrophage phenotypes were influenced by the culture medium; a murine macrophage-like cell line, J774.1/JA-4, expresses different activated-macrophage phenotypes induced by lipopolysaccharide (LPS) and/or interferon-γ (IFN-γ) when the cells are incubated in either Ham’s F-12 medium (F-12) or Dulbecco’s modified Eagle medium (DMEM). Among these phenotypes, NO production and iNOS expression are the most remarkably influenced by the medium; the induction of iNOS mRNA and iNOS protein is higher in DMEM than in F-12, but NO production by activated macrophages is less in DMEM than in F-12. These results suggest that the interpretation of the experimental results requires consideration of the possibility that the differences obtained by different laboratories were caused by the culture medium used.


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