alexa

GET THE APP

Interleukin-18 has an Important Role in Differentiation and Maturation of Mucosal Mast Cells | OMICS International| Abstract

Journal of Mucosal Immunology Research
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)
  • Research Article   
  • J Mucosal Immunol Res 2018: 109,

Interleukin-18 has an Important Role in Differentiation and Maturation of Mucosal Mast Cells

Nathan L Sandersa, Sathisha Upparahalli Venkateshaiah, Murli Manohar, Alok K Verma, Hemanth K Kandikattu and Anil Mishra*
Department of Medicine, Tulane Eosinophilic Disorders Center, Tulane University School of Medicine, New Orleans, USA
*Corresponding Author : Anil Mishra, Department of Medicine, Section of Pulmonary Diseases, Tulane Eosinophilic Disorders Center, Tulane University School of Medicine, New Orleans, LA, USA, Tel: 504-988-3840, Fax: 504-988-0647, Email: [email protected]

Received Date: May 30, 2018 / Accepted Date: Jun 25, 2018 / Published Date: Jul 02, 2018

Abstract

A significant amount of correlational evidence has linked increased levels of IL-18 with allergic diseases in both human and animal models, and, as mast cells are major mediators of allergies, we hypothesized that IL-18 may have a role in mast cell biology. Rationale for our hypothesis is based on the evidence that IL-3 deficient mice are not devoid of mast cells, even though IL-3 is a major differentiation and growth factor for mast cells. Accordingly, we cultured IL-18 responsive bone marrow CD34+ cells in vitro under a variety of conditions and cytokine combinations to examine mast cell differentiation and maturation using flow cytometry, quantitative PCR,and immunostaining techniques. Additionally, in vivo mast cell transformation and maturation were also analysed using endogenous IL-18 gene-deficient or Fabpi-IL-18 overexpressed mice. Our data indicate that both IL-3 and IL-18 exposed CD34+ bone marrow precursors differentiate and mature into mast cells. Further, we observed that IL-18 differentiates mast cells independent of IL-3, as pharmacologic blockade of IL-3 does not prevent in vitro IL-18-driven mast cell differentiation. Further, we found that endogenous IL-18 deficiency restricts maturation of IL-3 generated mast cells and IL-18 derived mast cells require IL-3 for their survival. Additionally, we observed IL-18 intestinal overexpression promotes tissue mast cell proliferation and mucosal mast cell development. Taken together, we provide the evidence that IL-18 has an important contributory role in mast cell differentiation, maturation and in vivo development of mucosal mast cells. Therefore, IL-18 may represent a future pharmacologic target for treating mast cell-mediated allergic diseases.

Keywords: Allergy; Interleukin-18; Mast cell; Mucosal immunity

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Article Usage
  • Total views: 1813
  • [From(publication date): 0-2018 - Feb 27, 2021]
  • Breakdown by view type
  • HTML page views: 1654
  • PDF downloads: 159
Top