Journal of Gastrointestinal & Digestive System
Open Access

Involvement of Fas receptors (CD95) and ligands (CD95L) in CD4+ T-cells and, CD8+ T-cells depletion and hepatic cytolysis in patients with Chronic Viral Hepatitis B

Franklin Steve Azebaze Agueguia^1*, Paul Talla², Marie Claire Okomo Assoumou³, Graeme Brendon Jacobs⁴, Cedric Happi Mbakam¹, Elise Guiedem¹, Martha Tongo Mesembe¹, Emilia Lyonga¹ and George Mondinde Ikomey¹

¹Center for the Study and Control of Communicable Diseases (CSCCD), Faculty of Medicine and Biological Sciences (FMBS), University of Yaoundé I, Cameroon

²Department of Internal Medicine and Specialties, Faculty of Medicine and Biomedical Sciences (FMBS), Yaoundé General Hospital, University of Yaoundé I, Cameroon

³Department of Microbiology, Haematology, Parasitology and Infectious Diseases, Faculty of Medicine and Biomedical Sciences (FMBS), University of Yaoundé I, Cameroon

⁴Division of Medical Virology, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, South Africa

Received Date: Apr 25, 2020 / Accepted Date: Jul 02, 2020 / Published Date: Jul 09, 2020

Citation: Agueguia FSA, Talla P, Assoumou MCO, Jacobs GM, Mbakam CH et al. (2020) Involvement of Fas receptors (CD95) and ligands (CD95L) in CD4+ T-cells and, CD8+ T-cells depletion and hepatic cytolysis in patients with Chronic Viral Hepatitis B. J Gastrointest Dig Syst 10: 618.

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Copyright: © 2020 Agueguia FSA et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Chronic Viral Hepatitis B (VHB) is characterized by a progressive destruction of hepatocytes and T-lymphocyte depletion. The mechanisms of the CD95-CD95L signaling pathway during chronic VHB and cirrhotic process remains unclear. Our objective was to evaluate the involvement of the CD95-CD95L receptor-ligands in T-lymphocyte depletion and hepatic cytolysis in patients with chronic VHB. Methods: A cross-sectional study was conducted from September to December 2018, at the Yaoundé General Hospital, Cameroon. Four milliliters of blood were collected and analyzed. The CD95, CD95L levels and CD4+, CD8+ T-cell counts were performed by ELISA and Flow cytometry, respectively. The data were analyzed using EpiInfo 7.0 and GraphPad PRISM 5.0, with the significant threshold set at p ≤ 0.05 and a 95% confidence interval. Results: Of the 130 patients, 36 (27.7%) were cirrhotic, and 94 (72.3%) were non-cirrhotic. The plasma level of CD95 and CD95L were significaly elevated in cirrhotic patients, compared with non-cirrhotic patients (p < 0.001 and p = 0.001 respectively). CD4/CD8 ratios were lower in cirrhotic patients, compared with non-cirrhotic patients (p < 0.001). There were statistically significant correlations between CD95 and CD4+, between CD95 and CD8+, between CD95 and CD4/CD8 ratio, between CD95 and fibrosis scores and between CD95L and fibrosis score. Conclusion: CD95-CD95L could be involved in T-lymphocyte depletion and hepatic cytolysis during the pathogenesis of chronic VHB, and could be used as biomarkers for immunological and hepatic monitoring in patients with chronic VHB.

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