Isolation and Characterization of Microorganisms that Degrade DimethoateShivangi Singh and Priya R Iyer*
PG and Research Department of Biotechnology, Women`s Christian College, Chennai, Tamil Nadu, India
- *Corresponding Author:
- Priya R Iyer
PG and Research Department of Biotechnology
Women`s Christian College, Chennai, Tamil Nadu, India
Tel: +91 044 2827 5926
E-mail: [email protected]
Received Date: August 28, 2017; Accepted Date: September 08, 2017; Published Date: September 11, 2017
Citation: Shivangi S, Priya RI (2017) Isolation and Characterization of Microorganisms that Degrade Dimethoate. J Bioremediat Biodegrad 8:410. doi: 10.4172/2155-6199.1000410
Copyright: © 2017 Shivangi S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Microorganisms have the ability to degrade a large number of pollutants found in the environment. Microorganisms such as bacteria can degrade toxic compounds and chemicals in an environment friendly way. Dimethoate is an organophosphate pesticide. It is a broad-spectrum insecticide. In the present study, dimethoate degrading microorganisms have been isolated from various soil samples. The different isolates that were obtained were characterized by various biochemical tests. Standardization of degradation was performed. Leuco crystal violet reagent was used as an indicator and colorimetric analysis was done at 595 nm. In total, 8 isolates were got. 3 belonged to Bacillus sp. and 3 were identified as Staphylococcus aureus. 2 isolates were identified as Aspergillus sp. HPLC analysis was carried out for the organism that showed the best degradation results.