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Hindi Research Article
Isolation and Evaluation of PAH Degrading Bacteria
| Raden Darmawan1,3*, Haruhiko Nakata1, Hiroto Ohta1, Takuro Niidome1, Kiyoshi Takikawa2 and Shigeru Morimura1 | ||
| 1Graduate School of Science and Technology, Kumamoto University, Kumamoto, Japan | ||
| 2Center for Marine Environment Studies, Kumamoto University, Kumamoto, Japan | ||
| 3Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember (ITS), Surabaya, Indonesia | ||
| Corresponding Author : | Raden Darmawan Graduate School of Science and Technology Kumamoto University Kumamoto, Japan Tel: +81-96-342-3669 Fax:+81-96-342-3669 E-mail: rdarmawan09@gmail.com |
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| Received February 23, 2015; Accepted March 24, 2015; Published March 27, 2015 | ||
| Citation: Darmawan R, Nakata H, Ohta H, Niidome T, Takikawa K, et al. (2015) Isolation and Evaluation of PAH Degrading Bacteria. J Bioremed Biodeg 6:283. doi:10.4172/2155-6199.1000283 | ||
| Copyright: © 2015 Darmawan R, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | ||
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Abstract
Polycyclic aromatic hydrocarbons (PAHs) degrading bacteria can be isolated from polluted coastal environments. They were close to Burkholderia fungorum and Mycobacterium gilvum, and had nidA, nidA3, pdoA2, and pcaH genes. In the pyrene and fluoranthene degradation experiment using a minimum medium, B. fungorum isolate no. 1 and M. gilvum isolate no. 13 were able to degrade 98.6% + 1.9 of pyrene after 20 days, and 99.9% ± 0.1 after a 16-day incubation period, respectively. Moreover, fluoranthene could be consumed by B. fungorum isolate no. 1 and M. gilvum isolate no. 13 at the level of 99.6% ± 0.7 after 24 days, and 100% after a 28-day incubation period, respectively.
