alexa
Reach Us +32 25889658
Metabolism and Preferential Utilization of Phenylacetic acid and 4-Hydroxyphenylacetic Acid in Pseudomonas putida CSV86 | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Metabolism and Preferential Utilization of Phenylacetic acid and 4-Hydroxyphenylacetic Acid in Pseudomonas putida CSV86

Rahul Shrivastava1, Hemant Purohit2 and Prashant S Phale1*
1Department of Biosciences and Bioengineering, Indian Institute of Technology-Bombay, Powai, Mumbai 400076, India
2Environmental Genomics Unit, National Environmental Engineering Research Institute, Nagpur-440020, India
Corresponding Author : Dr. Prashant S Phale
Department of Biosciences and Bioengineering
Indian Institute of Technology-Bombay
Powai, Mumbai 400076, India
Tel: +91-22-2576 7836
Fax: +91-22-2572 3480
E-mail: [email protected]
Received March 29, 2011; Accepted June 20, 2011; Published June 21, 2011
Citation: Shrivastava R, Purohit H, Phale PS (2011) Metabolism and Preferential Utilization of Phenylacetic acid and 4-Hydroxyphenylacetic Acid in Pseudomonas putida CSV86. J Bioremed Biodegrad 2:120. doi:10.4172/2155-6199.1000120
Copyright: © 2011 Shrivastava R. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google

Abstract

Metabolic pathways for phenylaceticacid (PA) and 4-hydroxyphenylacetic acid (4-HPA) from Pseudomonas putida CSV86 were elucidated. PA grown strain CSV86 cells showed higher oxygen-uptake on PA as compared to hydroxyphenylacetic acids. Detection of phenylacetyl-CoA ligase and absence of 4-hydroxyphenylacetic acid hydroxylase (4HPAH) and 3,4-dihydroxyphenylacetic acid-dioxygenase (3,4-DHPADO) activity supports this observation. 4-HPA grown cells showed oxygen-uptake on 4-HPA and 3,4-dihydroxyphenylacetic acid (3,4-DHPA) but showed significantly lower respiration rates on 2,5-dihydroxyphenylacetic acid and PA. Detection of 4HPAH and 3,4-DHPADO activities support the respiration data. Metabolic studies indicate that strain CSV86 metabolizes PA via phenylacetyl-CoA while 4-HPA via 3,4-DHPA pathway. Glucose grown cells showed lower activity of enzymes and respiration on PA, 4-HPA and 3,4-DHPA, suggesting that pathways are inducible. When grown on double carbon sources such as PA or 4-HPA plus glucose, CSV86 cells showed diauxic growth pattern with oxygen uptake on PA, 4-HPA and 3,4-DHPA in the first log-phase which was reduced during the second log-phase with increase in the respiration rate on glucose. This observation was supported by detection of high 4HPAH and 3,4-DHPADO activity in the first log-phase and glucose-6-phosphate dehydrogenase activity in the second log-phase. These results suggest that strain CSV86 utilizes PA and 4-HPA preferentially over glucose.

Recommended Conferences

2nd World Congress on Biopolymers & Bioplastics

Paris, France

4th Annual Congress on Soil, Plant and Water Sciences

Barcelona, Spain
Share This Page
Top