alexa Microbial Decolorization and Degradation of Orange 16 Dye by a Newly Isolated <em>Aeromonas Spp.</em> Etl-1949 | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
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Research Article

Microbial Decolorization and Degradation of Orange 16 Dye by a Newly Isolated Aeromonas Spp. Etl-1949

Maulin P Shah1*, Patel KA1, Nair SS1, Darji AM1 and Maharaul SJ2
1Industrial Waste Water Research Laboratory, Applied & Environmental Microbiology Lab, Enviro Technology Limited (CETP) Plot No: 2413/2414, GIDC, Ankleshwar- 393002, Gujarat, India
2Laboratory of Environmental Bioremediation, Narmada Clean Tech Limited, Final Effluent Treatment Plant, Surti Bhagor, Near Gujarat Gas, Umarvada Road, Ankleshwar-393001, Gujarat, India
Corresponding Author : Maulin P Shah
Industrial Waste Water Research Laboratory
Applied & Environmental Microbiology Lab
Enviro Technology Limited (CETP) Plot No: 2413/2414
GIDC, Ankleshwar- 393 002, Gujarat, India
Tel: +91-90 999 65504
Fax No: +91-2646-250707
E-mail: [email protected]
Received June 08, 2013; Accepted July 22, 2013; Published July 24, 2013
Citation: Shah MP, Patel KA, Nair SS, Darji AM, Maharaul SJ (2013) Microbial Decolorization and Degradation of Orange 16 Dye by a Newly Isolated Aeromonas Spp. Etl-1949. J Bioremed Biodeg 4:194. doi:10.4172/2155-6199.1000194
Copyright: © 2013 Shah MP, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

A bacterial strain ETL-1949, identified as Aeromonas spp. was isolated from sediment from a textile wastewater treatment plant in Ankleshwar, Gujarat, India. The strain could decolorize an azo dye, Orange 16 under the static condition after the shaking culture. In order to increase cell concentration by the shaking culture, the optimum culture condition was investigated for following the decolorization incubation. The dye was decolorized completely up to concentration of 750 mg/l for 50 hr under static condition by using 12 hr-cultured cells at optimal shaking culture condition. Effect of initial cell mass showed that higher cell mass concentration can accelerate decolorization process with maximum of 92% decolorization observed at 2.5 g/l cell mass within 6.5 h. Effect of various metal ions showed Mn & Mg has positive inducing effect whereas Zn strongly inhibited the decolorization process at 5 mM concentration. Analysis of biodegradation products carried out with UV–vis spectroscopy, HPTLC and FTIR confirmed the decolorization and degradation of Orange 16.

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