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ISSN: 2476-2024

Diagnostic Pathology: Open Access
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  • Research Article   
  • Diagn Pathol Open 2016, Vol 1(3): 116
  • DOI: 10.4172/2476-2024.1000116

Mir-27a: A Regulator of Apaf-1, Modulate Proliferation and Apoptosis in Laryngeal Carcinoma

Xu Ji1,2, Xiao-Wen Zhang1, Ning Liu3, Guang-Bin Qiu4*, Zhen-Ming Xu5* and Wei-Neng Fu1*
1Department of Medical Genetics, China Medical University, Shenyang, 110122, PR China
2Department of Otolaryngology, the First Affiliated Hospital of China Medical University, , Shenyang, 110001, PR China
3Department of Pancreatic Surgery, the First Affiliated Hospital of China Medical University, , Shenyang, Liaoning, China
4Department of Laboratory Medicine, No. 202 Hospital of PLA, , Shenyang, 110003, PR China
5Department of Otolaryngology, , No. 463 Hospital of PLA, Shenyang, 110007, PR China
*Corresponding Author (s) : Guang-Bin Qiu, Department of Laboratory Medicine, No. 202 Hospital of PLA, Shenyang, 110003, PR China
Zhen-Ming Xu, Department of Otolaryngology, No. 463 Hospital Of PLA, Shenyang, 110007, PR China
Wei-Neng Fu, Department of Medical Genetics, China Medical University, Shenyang, 110122, PR China, Tel: 024-23256666-5324, Email: [email protected]

Received Date: Jun 07, 2016 / Accepted Date: Jun 21, 2016 / Published Date: Jun 24, 2016

Abstract

Purpose: APAF-1, a key mediator in cytochrome C-dependent apoptotic pathway, plays a critical role in many cancers. MicroRNA-27a inhibits hypoxia-induced neuronal apoptosis by targeting APAF-1. Whether microRNA-27a participates in carcinogenesis via regulating APAF-1 is not reported.

Methods: Laryngeal cancer tissues, Hep2 cell line and HEK293 cell line were used in the study. qRT-PCR was used to detect microRNA-27a and APAF-1 mRNA levels. Western blot was to monitor APAF-1 protein level. Cell viability, colony formation and apoptosis assays were applied to evaluate the function of microRNA-27a and APAF-1 in laryngeal cancer. Dual-luciferase reporter assay was to detect the binding ability of microRNA-27a to APAF-1 3'UTR.

Results: In the study, we found that up-regulation of microRNA-27a was negatively correlated with downregulation of APAF-1 in laryngeal cancer. MicroRNA-27a was reconfirmed to directly bind APAF-1 mRNA 3'UTR. Similar to si-APAF-1, ectopic miR-27a significantly promoted laryngeal cancer cell proliferation and colony formation ability and suppressed early apoptosis compared to the controls.

Conclusion: miR-27a acts as a potentially oncogenic role in laryngeal squamous cell carcinoma partly though repressing APAF-1 expression, which enriches regulatory network of APAF-1 mediating apoptotic pathway.

Keywords: Laryngeal cancer; Mir-27a; Apaf-1; Proliferation; Apoptosis

Citation: Ji X, Zhang XW, Liu N, Qiu GB, Xu ZM, et al. (2016) Mir-27a: A Regulator of Apaf-1, Modulate Proliferation and Apoptosis in Laryngeal Carcinoma. Diagn Pathol Open 1: 116. Doi: 10.4172/2476-2024.1000116

Copyright: ©2016 Ji X. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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