alexa Molecular Methods for Detection of Beta-and Gamma-Papillomaviruses: A Review
ISSN: 2332-0877

Journal of Infectious Diseases & Therapy
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Review Article

Molecular Methods for Detection of Beta-and Gamma-Papillomaviruses: A Review

Alltalents T Murahwa1,2*, Monalisa T Manhanzva1,2, and Babill Stray-Pedersen3

1Department of Medical laboratory Sciences, University of Zimbabwe College of Health Sciences, Avondale, Harare, Zimbabwe

2Institute for Infectious Diseases and Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Division of Medical Virology, Cape Town, South Africa

3Institute of Clinical Medicine and Division of Obstetrics and Gynecology, Rikshospitalet, Oslo, University Hospital, Norway

*Corresponding Author:
Alltalents T Murahwa
Department of Medical laboratory Sciences
University of Zimbabwe College of Health Sciences
Avondale, Harare
Zimbabwe
Tel: 00263-779-136342
E-mail: [email protected]

Received date: June 27, 2016; Accepted date: July 08, 2016 ; Published date: July 13, 2016

Citation: Murahwa AT, Manhanzva MT, Stray-Pedersen B (2016) Molecular Methods for Detection of Beta-and Gamma-Papillomaviruses: A Review. J Infect Dis Ther 4: 289. doi:10.4172/2332-0877.1000289

Copyright: © 2016 Murahwa AT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

Accumulating evidence on beta and gamma Human Papillomavirus (HPV) genera, show its association with nonmelanoma skin cancers (NMSC). Current methods for detection of HPV in clinical specimens are molecular based. A variety of molecular techniques developed for diagnosis of HPV; focus more on detection of mucosal HPV types. A limited number of assays have been developed to detect and genotype cutaneous HPVs. These assays detect a proportion of the HPV types implicated in skin lesions with differing sensitivities and specificities. Conflicting literature on the prevalence of HPV in NMSC has resulted from the inconsistent diagnostic standards. This review discusses the methods available for detection and genotyping of HPVs with emphasis on cutaneous HPV genotypes. This information will provide researchers the relevant choices of molecular methods applicable to epidemiological surveys. The ultimate objective is to make automated, rapid and cheaper molecular approaches for resource poor settings.

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