alexa Nano Scale Potentiometric and Spectrophotometric Assays for 2,4-Dichlorophenoxyacetic Acid
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Nano Scale Potentiometric and Spectrophotometric Assays for 2,4-Dichlorophenoxyacetic Acid

El-Beshlawy MM*

Department of Chemistry, Collage of Girls, Ain Shams University, Egypt

*Corresponding Author:
El-Beshlawy MM
Department of Chemistry
Collage of Girls, Ain Shams University, Egypt
Tel: 00966538326107
E-mail: [email protected]

Received date: June 28, 2015; Accepted date: July 14, 2015; Published date: July 21, 2015

Citation: El-Beshlawy MM (2015) Nano Scale Potentiometric and Spectrophotometric Assays for 2,4-Dichlorophenoxyacetic Acid. J Anal Bioanal Tech 6:257 doi: 10.4172/2155-9872.1000257

Copyright: © 2015 El-Beshlawy MM. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The aim of this work was to investigate the ability of silicate wire ion selective electrode to measure 2,4 dichlorophenoxyacetic acid (2,4-D). The slope of the electrode was 56.2 mV decade -1 in the range from 10-6 to 10-2 M with lower limit of detection 8.3 × 10-7 M. The electrodes were successfully applied to determination of 2,4-D by separate and standard addition potentiometry. The electrode was used to detect the effect of pH and temperature on 2,4-D placed on baby watercress plant grown in the clay soil and the residue after five day from addition. Leaf chlorophylls quantity measured utilize spectrophotometric or colorimetric assay following extraction of pigments of watercress by ethanol. This work was carried at 286, 440, 662 nm. Beer’s law was obeyed in the concentration range of 2.21 mg/ml:2.21 μg/ml. The limits of detection were 2.21 μg/ml.

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