Observation of Viable Nontypeable Haemophilus Influenzae Bacteria within Neutrophil Extracellular Traps in Clinical Samples from Chronic Otitis MediaLauren B King1*, Bing Pang1*, Antonia C Perez1, Jennifer L Reimche1, Daniel J Kirse2, Amy S Whigham2, Adele K Evans2, and W Edward Swords1*
#Authors contributed equally
- Corresponding Author:
- Edward Swords W
Departments of Microbiology and Immunology
Wake Forest School of Medicine
Winston-Salem, NC 27101,USA
E-mail: [email protected]
Received date: September 06, 2013; Accepted date: October 01, 2013; Published date: October 09, 2013
Citation: King LB, Pang B, Perez AC, Reimche JL, Kirse DJ, et al. (2013) Observation of Viable Nontypeable Haemophilus Influenzae Bacteria within Neutrophil Extracellular Traps in Clinical Samples from Chronic Otitis Media. Otolaryngology 3:145. doi:10.4172/2161-119X.1000145
Copyright: © 2013 King LB, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Bacterial otitis media is an inherently inflammatory condition and often features an influx of neutrophils and other phagocytes into the middle-ear chamber. Neutrophils undergo a specific death pathway that results in formation of Neutrophil Extracellular Traps or “NETs”, which have been postulated as an antimicrobial defense mechanism that both mediates direct bacterial killing and facilitates phagocytic uptake and killing. However, we have shown that some mucosal pathogens within the airway including nontypeable Haemophilus influenzae survive within NETs in animal and in vitro models. In this report, we utilize exudate samples obtained from patients with chronic otitis media to show that nontypeable H. influenzae bacteria survive within NET structures within human patients.