Physiologic Behavioural Changes of Precancerous Leukoplakia Cell lines Exerted to Extremely Low Frequency Electric Field
- Corresponding Author:
- Wilhelm Niedermeier
Department of Prosthetic Dentistry
Dental School, University of Cologne
Kerpenerstr. 32, 50931 Cologne, Germany,
E-mail: [email protected]
Received Date: November18, 2014, Accepted Date: December 26, 2014, Published Date: December 31, 2014
Citation:Ahmed Korraah, Margarete Odenthal, Marion Kopp, Nadarajah Vigneswaran, Peter G Sacks, et al. (2015) Physiologic Behavioural Changes of Precancerous Leukoplakia Cell lines Exerted to Extremely Low Frequency Electric Field. J Interdiscipl Med Dent Sci 3:165. doi: 10.4172/2376-032X.1000165
Copyright: © 2015 Korraah A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objectives: Oral galvanism arising from the presence of two different metallic fillings in the mouth that may reach up to 16V/m, induces several changes in oral environment such as gingival swelling and erythema, mucosal pain, lichenoid reactions and leukoplakia.
Study design: To investigate in vitro the physiologic reactions of oral leukoplakia caused by electrostatic corrosion potentials, human leukoplakia cell lines (MSK-LEUK1), cultivated in KGM-2 supplemented with bullet kit in 5% CO2 humidified air at 37°C, were exposed to electric field strength of 1-20 V/m for 24 hours in a pseudo realistic apparatus called “Pulse chamber”. Following this, the cells were analysed for proliferation using BrdU assay, and for apoptosis using TUNEL assay. Findings were assessed utilizing fluorescent microscopy. Ultra structural changes were studied by TEM. Data was evaluated statistically using non parametric Chi-square test.
Results: Electric field strength of 1-10V/m led to upregulation of cell proliferation rate between 10.64% and 44.06% (p=0.0001). The apoptotic index increased significantly (p=0.0001) from 20.03% at 1V/m to 46.56% at 10V/m. Leukoplakia cells treated with 16V/m show individual cell keratinization.
Conclusion: Oral galvanism induces subcellular changes in oral precancerous leukoplakia cells in vitro that resemble some of the histopathologic features of oral squamous cell carcinoma cells in vivo.