Potential Effect of Two Bacillus spp on Decolorization of Azo dye | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

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Research Article

Potential Effect of Two Bacillus spp on Decolorization of Azo dye

Maulin P Shah*, Patel KA, Nair SS, Darji AM
Industrial Waste Water Research Laboratory, Division of Applied and Environmental Microbiology Lab, Enviro Technology Limited, Plot No: 2413/14, GIDC, Ankleshwar-393002, Gujarat, India
Corresponding Author : Maulin P Shah
Applied and Environmental Microbiology Lab
Enviro Technology, Limited (CETP)
Ankleshwar- 393 002, Gujarat, India
Tel: +91-90 999 65504
Fax No: +91-2646-250707
E-mail: [email protected]
Received July 15, 2013; Accepted August 20, 2013; Published August 22, 2013
Citation: Shah MP, Patel KA, Nair SS, Darji AM (2013) Potential Effect of Two Bacillus spp on Decolorization of Azo dye. J Bioremed Biodeg 4:199. doi: 10.4172/2155-6199.1000199
Copyright: © 2013 Shah MP, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Decolorization of azo dye by two bacterial species Bacillus megaterium and Bacillus cereus has been analyzed using mineral effluent, consisting of known concentration of the dye in ZZ medium. Physico chemical parameters such as carbon and nitrogen source, temperature, pH and inoculum volume are optimized for the decolorization process by changing one parameter at a time. Optimal condition for Bacillus cereus was found to be 1% sucrose, 0.25% peptone, pH 7, temperature 37°C and 8% inoculum and that for Bacillus megaterium was found to be glucose 1%, 0.25% yeast extract, pH 6, temperature 37°C and 10% inoculum. Extent of decolorization recorded by Bacillus cereus under ideal conditions was 95% and that by Bacillus megaterium was 98%. The study has confirmed the potential of Bacillus cereus and Bacillus megaterium in the decolorization of Azo dye and opened scope for future analysis of their performance in the treatment of textile effluent.