Quantification of Pharmacologically Active Markers Gallic Acid, Quercetin and Lupeol from Acacia Leucophloea Wild Flowers by HPTLC Method
Leela V* and Saraswathy A
Captain Srinivasa Murti Drug Research Institute for Ayurveda (CCRAS), Chennai, Tamil nadu, India
- *Corresponding Author:
- Leela Vadivelu
Captain Srinivasa Murti Drug Research Institute for Ayurveda (CCRAS)
Tamil nadu, India
E-mail: [email protected]
Received date: January 01, 2013; Accepted date: January 28, 2013; Published date: February 05, 2013
Citation: Leela V, Saraswathy A (2013) Quantification of Pharmacologically Active Markers Gallic Acid, Quercetin and Lupeol from Acacia Leucophloea Wild Flowers by HPTLC Method. J Anal Bioanal Tech 4:160.doi: 10.4172/2155-9872.1000160
Copyright: © 2013 Leela V, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Objective: TLC densitometric method for quantification of gallic acid, quercetin and lupeol using HPTLC is developed. This is the first report of quantification of these three bioactive compounds viz. Gallic acid, quercetin and lupeol using HPTLC from this plant.
Methodology: Quantification of gallic acid and quercetin was carried out from the methanolic extract using the solvent system of Toluene:Ethyl acetate:Formic acid (6:4:0.8 v/v/v). Lupeol was quantified from chloroform extract using the solvent system of Toluene:Ethyl acetate (7:3 v/v).
Results: The Rf values of gallic acid, quercetin and lupeol are 0.22, 0.37 and 0.70 respectively. The linearity ranges for gallic acid (100 to 600 ng), quercetin (2000 to 7000 ng) and lupeol (100 to 1200 ng) with correlation coefficients (r-values) of 0.99968, 0.99708, and 0.99971 respectively. The amount of gallic acid, quercetin and lupeol was 124.31, 580.4, 24.89 μg/ml respectively.
Conclusion: Quantification of gallic acid, quercetin and lupeol showed good resolution and separation from other constituents of extract. Its main advantages are its simplicity, accuracy, and selectivity. This method can also be used for the estimation of these compounds in other herbal preparations and may be useful for standardization purposes.