Quantitative Method for the Determination of Posaconazole in Mouse Tissues using Liquid Chromatography-Mass SpectrometryIbrahim El-Serafi1, Tommy Pettersson2, Ola Blennow3,4, Jonas Mattsson3,4, Erik Eliasson2, Anton Pohanka2, and Moustapha Hassan1,5*
- *Corresponding Author:
- Moustapha Hassan
Experimental Cancer Medicine(ECM)
Clinical Research Centre (KFC)
Department of Laboratory Medicine
Karolinska Institutet Huddinge
Novum, 141 86 Stockholm, Sweden
Tel: +46-8-585 838 62
E-mail: [email protected]
Received date: June 02, 2014; Accepted date: June 26, 2014; Published June 28, 2014
Citation: El-Serafi I, Pettersson T, Blennow O, Mattsson J, Eliasson E, et al. (2014) Quantitative Method for the Determination of Posaconazole in Mouse Tissues using Liquid Chromatography-Mass Spectrometry. J Anal Bioanal Tech 5:193 doi: 10.4172/2155-9872.1000193
Copyright: © 2014 El-Serafi I, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Fast and selective liquid chromatography-mass spectrometry (LC-MS) method was developed for the quantification of posaconazole in different mouse organs including liver, heart, brain, kidney and lung. Organs were homogenized and diluted in isotonic NaCl solution. Protein was precipitated using acetonitrile containing internal standard and analyzed. The analysis was carried out using gradient condition with mobile phases consisting of aqueous formic acid and pure acetonitrile. Analysis was run at a flow-rate of 0.51 mL/min. The method was selective with a limit of quantification of 0.5 μg/mL in homogenate at a sample volume of 100 μL. The standard curve was linear over a concentration range of 0.5-10 μg/mL for all organs. The inter-day and intra-day coefficient of variation values were all less than 15%. Hence, the method is suitable for use in pharmacokinetic and bioavailability studies of posaconazole in tissues.