Journal of Analytical & Bioanalytical Techniques
Open Access

Research Article

Rapid Measurement of Adenosine Concentration in Human Blood Using Fixed Potential Amperometry: Comparison with Mass Spectrometry and High- Performance Liquid Chromatography

¹Laboratory of Biochemistry, Timone Hospital 264 Rue Saint-Pierre, 13385 Marseille, France

²UMR MD2, Aix Marseille University, Bd Pierre Dramard, 13015 Marseille, France

³RUSH Department, Timone 2, 264 Rue Saint-Pierre, 13385 Marseille, France

⁴Department of Cardiology, Timone Hospital 264 Rue Saint-Pierre, 13385 Marseille, France

*Corresponding Author:

Régis Guieu
UMR MD2, Aix Marseille University
Bvd P Dramard 13015, Marseille
France
Tel: +33491396500
E-mail: guieu.regis@orange.fr

Received date: July 11, 2017; Accepted date: July 17, 2017; Published date: July 20, 2017

Citation: Marlinge M, Vairo D, Marolda V, Bruzzese L, Adjriou N, et al. (2017) Rapid Measurement of Adenosine Concentration in Human Blood Using Fixed Potential Amperometry: Comparison with Mass Spectrometry and High-Performance Liquid Chromatography. J Anal Bioanal Tech 8: 371. doi: 10.4172/2155-9872.1000371

Copyright: © 2017 Marlinge M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Background: Adenosine is a nucleoside that impacts the cardiovascular system during cardiovascular or inflammatory diseases. The rapid determination of adenosine in blood may be useful in emergency medicine especially in syncope diagnose or septic shock. We compare its measurement in blood using fixed potential amperometry (FPA), with usual methods: mass spectrometry (LC-MS/MS) or high performance liquid chromatography (HPLC). Methods: Twenty healthy subjects (14 men and 6 women) and ten patients suffering from vasovagal syncope (VVS, 6 women and 4 men) were included. Blood samples were collected by vein puncture for plasma adenosine assay and in the same time using finger puncture for direct FAP measurement and on blotting paper for LC-MS/MS. Results: Mean plasma adenosine concentration was 26% higher using HPLC compared with LC-MSMS; p<0.01. In whole blood, adenosine concentration was 35% higher using FPA compared with LC-MS/MS. We found a good correlation between adenosine values measured by FAP and LC-MS/MS in whole blood and between LCMS/ MS and HPLC in plasma. Mean adenosine concentration was higher in patients whatever the method used. Conclusion: Adenosine measurement to the patient’s bed, using FPA may be useful in some cases where high adenosine is associated with pejorative outcome.

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