Simple Determination of Sugars in CigarettesEugene Jansen*, Johannes Cremers, Simon Borst and Reinskje Talhout
Centre for Health Protection, National Institute for Public Health and the Environment, Bilthoven, The Netherlands
- *Corresponding Author:
- Eugene Jansen
Centre for Health Protection
National Institute for Public Health and the Environment
PO Box 1, 3720 BA Bilthoven, The Netherlands
E-mail: [email protected]
Received date: October 24, 2014; Accepted date: November 07 2014; Published date: November 11, 2014
Citation: Jansen E, Cremers J, Borst S, Talhout R (2014) Simple Determination of Sugars in Cigarettes. J Anal Bioanal Tech 5:219 doi: 10.4172/2155-9872.1000219
Copyright: © 2014 Jansen E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Simple sugars are an important constituent of tobacco, varying between almost 0 and 20% on weight of tobacco. Some manufacturers add sugars to the natural content in tobacco. In the present report, a simple and fast method is presented to analyze the concentrations of the sugars sucrose, glucose and fructose in cigarettes. After an extraction step, the diluted samples are applied to an isocratic HPLC system with detection by evaporated light scattering (ELSD). The method is very stable and sensitive with a good reproducibility. In one working day 80 samples of cigarettes can be processed and prepared for overnight HPLC analysis by one technician. The average total sugar content in 58 commercial cigarette brands is 17.4% (w/w) with a range of 1.9 to 18.3%.