Simultaneous Estimation of Tenofovir and Emtricitabine in Human Plasma Using HPLC after Protein Precipitation Extraction
Arti Soni* and Seema Thakral
GVM College of Pharmacy, Murthal Road, Sonipat, India
- *Corresponding Author:
- Arti Soni
GVM College of Pharmacy
E-mail: [email protected]
Received date: May 22, 2013; Accepted date: August 05, 2013; Published date: August 07, 2013
Citation: Soni A, Thakral S (2013) Simultaneous Estimation of Tenofovir and Emtricitabine in Human Plasma Using HPLC after Protein Precipitation Extraction. J Anal Bioanal Tech 4:170. doi: 10.4172/2155-9872.1000170
Copyright: © 2013 Soni A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Tenofovir (TNF) and emtricitabine (FTC) are both reverse transcriptase inhibitors, often used in combination for anti-retroviral therapy. In the present study, a reverse phase high performance liquid chromatographic method was developed and validated for the simultaneous estimation of TNF and FTC in human plasma using stavudine as the internal standard. Protein precipitation extraction procedure utilizing perchloric acid was employed to extract the drugs from human plasma. Estimation of the drug contents was done by using a mixture of sodium dihydrogen orthophosphate buffer (pH 6.9) and methanol as the mobile phase and absorbance was read at 259 nm for TNF and 280 nm for FTC. Retention time was found to be 6.6 (± 0.1) min for TNF and 17.1 (± 0.2) min for FTC. The recovery, selectivity, linearity, precision as well as the accuracy of the method was evaluated from spiked human plasma samples during the course of validation. The result revealed that the analytical technique presented here demonstrates acceptable accuracy and precision, shorter and simpler sample preparation, and a reduced need for complicated equipment along with a tolerable analysis time.