Using <em>Chlorella vulgar</em> is to Decrease the Environmental Effect of Garbage Dump Leachates | OMICS International | Abstract
ISSN: 2155-6199

Journal of Bioremediation & Biodegradation
Open Access

Like us on:

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Using Chlorella vulgar is to Decrease the Environmental Effect of Garbage Dump Leachates

Sarunporn Thongpinyochai and Raymond J Ritchie*
Tropical Plant Biology, Faculty of Technology and Environment, Prince of Songkla University Phuket Campus, Kathu, Phuket 83120, Thailand
Corresponding Author : Raymond J Ritchie
Tropical Plant Biology, Faculty of Technology and Environment
Prince of Songkla University Phuket Campus
Kathu, Phuket 83120, Thailand
Tel: +6676 276130
Fax: +66 76 276102
E-mail: [email protected]
Received May 28, 2014; Accepted July 25, 2014; Published July 29, 2014
Citation: Thongpinyochai S, Ritchie RJ (2014) Using Chlorella vulgar is to Decrease the Environmental Effect of Garbage Dump Leachates. J Bioremed Biodeg 5:239. doi:10.4172/2155-6199.1000239
Copyright: © 2014 Ritchie RJ, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Related article at
DownloadPubmed DownloadScholar Google


Waste treatment in Phuket, Thailand produces two sources of leachate. The Landfill Leachate (LF) has low BOD (60-405 mg O2/L) and heavy metal levels are below legal limits. Garbage awaiting incineration, Garbage Pit Leachate (GPL), also produces a second leachate with a very high BOD (50-100 g O2/L) and NH3-N (763-2,045 mg/L). Zn and Cr exceed standards but Cu and Pb are low. Chlorella vulgaris was tested for its ability to decrease NH3-N, NO3--N, Total-P, BOD and COD and to remove and/or immobilize heavy metal at varying dilutions of leachate. The objectives of this study on Chlorella bioremediation were: 1) measure Chlorella growth in Landfill and Garbage Pit Leachate, 2) measure the effectiveness of Chlorella to decrease the physico-chemical parameters NH3- N, NO3-N, Total-P, BOD, COD 3) measure heavy metal removal from leachates. The minimum inoculum of Chlorella biomass which can grow in the LF was Chlorophyll a 0.259 μg/mL (A750=0.075). Chlorella significantly lowered its BOD and COD: Cr and Ni, already low in landfill leachate, were decreased by 70% and 66%. Chlorella grew well in LF diluted 30 % with tapwater: % removal of NH3-N (53.91%), BOD (52.78%) and COD (51.05%). The GPL was very toxic: only 10-20% dilutions of GPL were tolerated by Chlorella and cultures required continuous aeration by shaking to grow. The minimum necessary inoculum of Chlorella biomass in the GPL was Chlorophyll a=0.92 μg/mL (A750=0.19). In 20% dilution of GPL, Cr and Zn were decreased by 33% and 90% respectively to legal levels. % removal of NH3-N (41.5%), NO3--N (32.4%), Total-P (55.1%), BOD (49.2%) and COD (50.8%). Chlorella inoculations with a biomass of 1.17 μg/mL Chlorophyll a (A750=0.202) removed 90% of the Zn.