alexa Utility of Calretinin Stain in Work-up of Inadequate Biopies in Patient’s with Hirschsprung’s Disease
ISSN: 2161-069X

Journal of Gastrointestinal & Digestive System
Open Access

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Research Article

Utility of Calretinin Stain in Work-up of Inadequate Biopies in Patient’s with Hirschsprung’s Disease

Sikandar M*, Nagi AH and Naseem N

Department of Morbid Anatomy and Histopathology, University of Health Sciences, Lahore, Pakistan

Corresponding Author:
Mishal Sikandar
Department of Morbid Anatomy and Histopathology
University of Health Sciences, 96-Z
Street 32, Phase 3, DHA, Punjab 042, Lahore, Pakistan
Tel: 9203346508547
E-mail: [email protected]

Received Date: June 08, 2017; Accepted Date: July 05, 2017; Published Date: July 12, 2017

Citation: Sikandar M, Nagi AH, Naseem N (2017) Utility of Calretinin Stain in Work-up of Inadequate Biopies in Patient’s with Hirschsprung’s Disease. J Gastrointest Dig Syst 7:515. doi:10.4172/2161-069X.1000515

Copyright: © 2017 Sikandar M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.



Background and Objective: A thorough study was designed with an objective of observing utility of calretinin as immune-histochemical marker for aganglionosis and for detection of ganglion cells in the inadequate biopies of affected areas for more accurate and better diagnosis of the disease.

Study Design: It was observational, descriptive study.

Setting: It was carried out at the Department of Morbid Anatomy and Histopathology in University of Health Sciences (UHS) Lahore.

Period: The study commenced in March 2016 after approval of the synopsis by the Advance Studies and Research Board of UHS and was successfully completed in December 2016.

Methodology: Biopsy specimens of colon which were considered for the study were collected from 73 patients from Mayo Hospital and Jinnah Hospital, Lahore with established histopathologically diagnosed HSCR on H&E staining.

Results: The mean age was 12.52 ± 9.21 months. On the basis of Calretinin staining Ganglion cells were present in 42/73 (57.53%) and absent in 31/73 (42.47%) respectively.

Conclusion: It was consummated that Calretinin provides a reliable and very cost effective adjunctive test to be used routinely with H&E in diagnosing HSCR and consequently waiving off the need for unnecessary surgeries and repeated biopsies.


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