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Validated HPTLC Method for Simultaneous Estimation of Metformin Hydrochloride, Atorvastatin and Glimepiride in Bulk Drug and Formulation | OMICS International | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
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Research Article

Validated HPTLC Method for Simultaneous Estimation of Metformin Hydrochloride, Atorvastatin and Glimepiride in Bulk Drug and Formulation

Sunil R. Dhaneshwar*, Janaki V. Salunkhe and Vidhya K. Bhusari

Department of Pharmaceutical Chemistry, Bharati Vidyapeeth University, Poona College of Pharmacy, Pune, Maharashtra, India

*Corresponding Author:
Dr. Sunil R. Dhaneshwar
Department of Pharmaceutical Chemistry
Bharati Vidyapeeth University, Poona College of Pharmacy
Pune, Maharashtra, India
Tel: (+91-20) 25437237
Fax: (+91-20) 25439383
E-mail: [email protected]

Received date: October 12, 2010; Accepted date: November 22, 2010; Published date: November 24, 2010

Citation: Dhaneshwar SR, Salunkhe JV, Bhusari VK (2010) Validated HPTLC Method for Simultaneous Estimation of Metformin Hydrochloride, Atorvastatin and Glimepiride in Bulk Drug and Formulation. J Anal Bioanal Tech 1:109. doi:10.4172/2155-9872.1000109

Copyright: © 2010 Dhaneshwar SR, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

This paper describes a new, simple, precise, and accurate HPTLC method for simultaneous estimation of Metformin hydrochloride (MET), Atorvastatin (ATV) and Glimepiride (GLM) as the bulk drug and in tablet dosage forms. Chromatographic separation of the drugs was performed on aluminum plates precoated with silica gel 60 F 254 as the stationary phase and the solvent system consisted of water: methanol: ammonium sulphate (1: 1: 4 v/v/v). Densitometric evaluation of the separated zones was performed at 237 nm. The three drugs were satisfactorily resolved with R F values 0.37 ± 0.02 and 0.59 ± 0.02, 0.75 ± 0.02 for MET, ATV, GLM respectively. The accuracy and reliability of the method was assessed by evaluation of linearity (200-700 ng/spot for MET, 600-2100 ng/spot for ATV and 600-2100 ng/spot for GLM ), precision (intra-day% RSD was 0.54–1.23 and inter-day% RSD was 0.90–1.48 for MET, intra-day% RSD was 0.91–1.74 and inter-day% RSD was 0.56–1.52 for ATV and intra-day% RSD was 0.60–1.27 and inter-day% RSD was 0.96–1.48 for GLM ), accuracy (99.66 ± 0.14 for MET, 98.46 ± 0.40 for ATV and 98.62 ± 0.39 for GLM ), and specificity in accordance with ICH guidelines.

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