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Hindi Research Article
Yeast Extract as the Most Preferable Substrate for Optimized Biosurfactant Production by rhlB Gene Positive Pseudomonas putida SOL-10 Isolate
| Muneer Ahmed Qazi1, Zulfiqar Ali Malik1,2, Ghazi Dino Qureshi1, Abdul Hameed1 and Safia Ahmed1* | |
| 1Department of Microbiology, Faculty of Biological Sciences, Quaid-i-Azam University, Islamabad 45320, Pakistan | |
| 2Department of Microbiology, Faculty of Natural Sciences, Shah Abdul Latif University, 66111 Khairpur Mir’s, Sindh-Pakistan | |
| Corresponding Author : | Safia Ahmed Department of Microbiology Faculty of Biological Sciences Quaid-i-Azam University Islamabad 45320, Pakistan Tel: +9251-90643009 E-mail: safiamrl@yahoo.com |
| Received: June 09, 2013; Accepted: September 21, 2013; Published: September 27, 2013 | |
| Citation: Qazi MA, Malik ZA, Qureshi GD, Hameed A, Ahmed S (2013) Yeast Extract as the Most Preferable Substrate for Optimized Biosurfactant Production by rhlB Gene Positive Pseudomonas putida SOL-10 Isolate. J Bioremed Biodeg 4:204. doi:10.4172/2155-6199.1000204 | |
| Copyright: © 2013 Qazi MA, et al. This is an open-a ccess article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. | |
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Abstract
Oil contaminated sites are enriched source of microorganisms that produce a variety of surface active amphiphilic compounds known as biosurfactants. Pseudomonas putida SOL-10 strain isolated from oil contaminated soil of Fimkassar oil field, Chakwal, Pakistan, was identified by standard morphological, biochemical and 16S rRNA sequence analysis methods. SOL-10 strain was initially screened for biosurfactant production using oil spreading test and then manifestation of rhlB (rhamnolipid) gene was confirmed by PCR using gene-specific primers. Maximum biosurfactant production in terms of surface tension (29.9 mN m-1) and emulsification index (E24, 73.45%), was achieved when the strain was grown in MSM supplemented with yeast extract (1.5-2 %, w/v) and urea (0.1 %, w/v) as carbon and nitrogen sources, respectively, and the physical parameters were adjusted at pH 7.0, temperature 30°C, 150 rpm agitation speed. The biosurfactant emulsified various hydrocarbons tested, being more effective against xylene and kerosene (85.19% and 70.59%, respectively). The crude biosurfactant also showed stability at a wide range of temperature (25-80°C), pH (1-9) and salt concentration (1-5%, w/v). The stability and hydrocarbon emulsifying potential of the biosurfactant indicated its possible use as decent contender for future environmental applications like biodegradation and bioremediation of organic pollutants.
