alexa Breeding of Sistan Yaghouti Grape using Clonal Selection | OMICS International
ISSN: 2155-952X
Journal of Biotechnology & Biomaterials

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Breeding of Sistan Yaghouti Grape using Clonal Selection

Maryam Allahdou1*, Abbasali Nouri Sadegh2 and Shirali Kouhkan3

1Department of Plant Breeding and Biotechnology, University of Zabol, Zabol, Iran

2Iran-Azad University, Zabol, Iran

3Iran-Center of Zabol Agriculture and Resource Natural Research, Zabol, Iran

Corresponding Author:
Maryam Allahdou
Department of Plant Breeding and Biotechnology
University of Zabol, Zabol, Iran
E-mail: [email protected]

Received date: November 02, 2012; Accepted date: December 14, 2012; Published date: December 17, 2012

Citation: Allahdou M, Sadegh AN, Kouhkan S (2012) Breeding of Sistan Yaghouti Grape using Clonal Selection. J Biotechnol Biomaterial S13:004. doi:10.4172/2155-952X.S13-004

Copyright: © 2012 Allahdou M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Sistan Yaghouti grape belongs to the category of seedless grapes which are resistant to diseases and they have an uninterrupted growth in adverse conditions. Sistan weather usually has hot wind and salty soil. In order to increase the fruit quality, we investigated vine yards of Sistan areas such as Zahak, Dust-Mohammed and Bonjar and collected samples of Yaghouti grape. Traits of Yaghouti grape were calculated using Descriptor method. Results showed that Yaghouti grape clones had high variation in traits such as density of branch, size of berry, weight of berry, weight of branch and height of branch. Therefore, it can be said that clonal selection is suitable method for breeding of grape for good branch quality. Collected seedlings were also planted in Zahak agriculture research station for further evaluation and stability of traits with respect to location and season.


Yaghouti grapevine; Clonal selection; Sistan


Grapevine is an important horticulture plant in Sistan area which has been cultured currently. Important cultivar grape in Sistan are red Yaghouti, white Yaghouti, Fakhri, Sangak, Amiri, Laal, Haji Abbasi, Cow eye, Ghandehari, Mayemish, Gholami and Shastarous. Red Yaghouti grapevine belong to seedless grapes group which is resistance to diseases and grow in conditions of Sistan weather which usually have hot winds and salty soil, also they mature early and are harvested in June. So it is economically useful for Sistan people. But branches of this grape have low density with small berries and have low quality fruit.

In plant breeding methods, generally breeding method corresponds with the biological properties of the plant to be generated biologically. Breeding of fruit trees becomes very difficult because of their seasonal growth and longer growth periods. For effective investigation many cross breeds are required between the parents and subsequently a good number of progeny to examine upon the genetic basis of inheritance. Therefore, for multiannual plants such as fruit trees, clonal selection is useful [1]. Selection is process of choosing the best single plants from the heterogenic population. Efficiency of selection is related to content of genetic variation, heritability and kind of selection method [2].

According to breeders, a three stage evaluation system (A, B and C) for clonal selection is efficient. The three stage selection resulted in clones with high yield, free from diseases and production of homogeny yard vines [3].

Subject of this research was selection of red Yaghouti grapevine with large berry, low density and high yield.

Materials and Methods

In order to evaluate, Sistan red Yaghouti grapevines were collected from grapevines of different region of Sistan such as Zahak region, Jazinak region, Hirmand region and Bonjar region. From each region, they were randomly selected, and then the best trees were chosen and were signed. Fruits of these trees were harvested and plantlets from the trees were selected. Selected plantlets were transported to station of agriculture research located to Zahak and were cultured in pool. Traits such as length of branch, length of berry, weight of single branch, weight of berry, and density were calculated based on descriptor method. Analysis of data was calculated using SAS software version 9.1 [4].

Results and Discussion

Collected grapevines from the different region of Sistan showed high variability for the investigated traits. According to table 1, mean weight of 5 branches were variable. Mean weight of berry were in range of 4.26 to 7.43. Density of branch has direct relation with weight of berry and increase in weight of berry resulted in low density. Therefore, in order to breed Yaghouti grape and to decrease the branch density, these methods should be used which in turn increase the weight of berry. The vines shifted to Jazinak had almost high quality of branch density, weight of berry and length of berry. It seems that there is an inverse relationship between the length of the branch and cluster density. Vine yards which belong to Mr. Rigi had high density branches and small berry size. Vine yard which belong to Jahanbakhsh located in Hirmand region had low density of branch, large berry and were with high quality. Coefficient of correlation analysis showed that weight of berry had significant correlation with length of berry (0.641) and also with height of branch (0.542). Notzuka et al. [5] used induced polyploid grapes using in vitro chromosome and the resulting 4X grapes had bigger size of berry than 2X grapes. In the first stage, trees which had low density, height of branch and large berry were signed and selected and then plantlets were cultured for second stage selection. In the second stage selection, trees which will have high quality will be selected for experimental stability.

Area Weight
of branch
of berry
of berry
High branch
Zahak 144.15 6.44 8.77 12.33
Zahak 142.02 6.00 7.90 9.66
Zahak 120.23 5.36 7.52 10.56
Zahak 129.63 6.95 8.52 11.20
Zahak 130.65 7.12 8.85 11.69
Jazinak 135.13 7.43 9.11 12.00
Jazinak 123.92 5.77 7.72 11.00
Jazinak 120.93 5.85 7.96 10.68
Jazinak 142.32 6.25 8.21 09.21
Jazinak 120.45 5.98 7.26 11.26
Hirmand 130.58 7.26 9.45 11.78
Hirmand 118.95 4.26 6.64 10.29
Hirmand 120.96 5.26 6.28 10.59
Bonjar 125.36 5.96 6.89 11.36
Bonjar 128.96 6.23 6.68 11.56
Bonjar 130.26 6.86 6.28 11.23
Bonjar 134.26 5.89 6.29 10.86

Table 1: Means of investigating traits in Yaghouti grape.


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