Epidemiology of Bovine Mastitis in Ethiopia
Received Date: Jan 08, 2018 / Accepted Date: Jan 29, 2018 / Published Date: Feb 05, 2018
Mastitis is inflammation of the mammary gland, including not only intramammary tissues but also related anatomical structures such as nipples, mammary areolas, milk ducts. The occurrence of disease is an outcome of interplay between three major factors: infectious agents, host resistance, and environmental factors. It is considered to be one of the most important causes of economic losses in the dairy industries worldwide. Clinical Mastitis can be defined as a farmer’s observed abnormality in the milk and/or the udder. Cows are visibly sick, or the milk is visibly abnormal. Subclinical Mastitis is by far the costlier disease in the majority of herds, and is often defined as the presence of a microorganism in combination with an elevated somatic cell count (SCC) of the milk. In Ethiopia, the diseases have got a major on dairy industry and the prevalence of disease has been reported by several authors in different parts of Ethiopia, several of these studies have shown the occurrence of a range of Mastitis causing bacteria, indicating Staphylococcus, Escherichia coli and Streptococcus as dominant and pathogenic species. Even though the disease is insufficiently investigated and information relating to its magnitude, distribution and risk factors is scant, breed, age, parity, tick infestation of udder, sequence of milking, floor, husbandry system, lactation stage and hygiene of udder were reported to be important risk factors for the prevalence of bovine Mastitis in Ethiopia. The diagnostic tests commonly used in Ethiopia are: California Mastitis test (CMT), bacterial culture and isolation direct microscopy, indicator paper test. Mastitis remain the most devastating disease in livestock production. Especially, the sub-clinical Mastitis remains to be an obscure and latent form of this disease that poses more serious economic losses to the dairy livestock sector, as the incidence is much higher in a dairy herd than the clinical one. Therefore, the nature of the disease necessitates research, early detection and effective control programs to be under taken.
Keywords: Bovine Mastitis; Ethiopia; California Mastitis test; Clinical Mastitis; Subclinical Mastitis; Somatic cell count
Ethiopia, located in tropical region and the country is greatly dependent on Agriculture. Livestock production represents a major national resource and forms an integral part of the Agricultural production system and livelihood of the society. Ethiopia has the largest cattle population in Africa with an estimated population of 56.71 million . Among this cow represents the biggest portion of cattle population of the country, around 20.7% of the total cattle heads are milking cows . However, milk production often does not satisfy the country’s requirements due to a different of factors. Of these factors, Mastitis is one the factors contributing to reduced milk production . Mastitis is also associated with number of zoonotic diseases in which milk acts as a vehicle of infection .
Mastitis is defined as an inflammation of the parenchyma of mammary gland, which can reduce milk yield and alter milk composition . The occurrence of disease is an outcome of interplay between three major factors: Infectious agents, Host resistance, and Environmental factors . Mastitis in dairy cows occurs worldwide and can be caused by infections with bacteria, yeast and fungi . There are two main classes of Mastitis. The first is clinical Mastitis, which manifests signs observed from the animal or the milk. The other is Subclinical Mastitis, which produces no visible signs from the udder except when using diagnostic tools. Despite many years of research, subclinical Mastitis remains the most economically damaging and zoonotic potential disease for dairy industry and consumer worldwide irrespective of species of animal .
Mastitis is the common and costly disease causing loss in milk yield, treatment cost for dairy farmers and culling of animals at unacceptable age . Since the quality and quantity of the milk is influenced by Mastitis, it is considered to be one of the most important causes of economic losses in the dairy industry worldwide. Economic losses caused by Mastitis also include value of discarded milk .
Dairy enterprise is very gradual in countries of sub Saharan Africa like Ethiopia. In this region, the low local milk production is as result of many factors including low genetic potential for milk production of indigenous breeds, the extensive and low inputs husbandry practices under which they are reared and wide spread livestock diseases . According to CSA  in Ethiopia 11.33 million of total cattle for private holdings are milking cows; however, milk production often doesn’t satisfy the countries milk requirement due to multitude factors. Some authors  reported a substantial economic loss in Ethiopian highland crossbred dairy cows due to subclinical Mastitis.
Bovine Mastitis as a disease, has received little attention in Ethiopia, especially the Sub clinical form. When modern dairy farming in the tropics was first adopted Mastitis was predicted to be important disease in dairy cattle and one of the most tasks of risk factors for Animal health and production problems to allow effective control strategies to be adopted . The disease has been reported by several authors in different parts of Ethiopia [14-16]. However, still there is a gap in Ethiopia, the disease is insufficiently investigated and information relating to its magnitude, distribution and risk factors is scant. Such information is important to envisage when designing appropriate strategies that would help to reduce its prevalence and effects [16,17].
Several of these studies have shown the occurrence of a range of Mastitis causing bacteria, indicating Staphylococcus, Escherichia coli and Streptococcus as dominant and pathogenic species, clinical Mastitis is readily apparent and easily detected. In contrast, detection of mammary quarters with Sub-clinical Mastitis is more difficult because signs are not readily apparent  and because of the lack of any overt manifestation, its diagnosis is a challenge in dairy animal management and in Veterinary practice. There are different levels for detection of Mastitis: an individual cow level in the herd, and a more large-scale testing for bulk milk . Regarding the individual cow level, the sub-clinical form of the disease can be detected by bacteriological examination and somatic cell counts (SCC) . Under field conditions, determination of SCC in milk is usually done using the California Mastitis test (CMT); in fact, CMT scores are directly related to average SCC [20,21]. Thus, the objective and significance of this paper is to review the general status of Bovine Mastitis and increase the awareness of the importance of disease in Ethiopia.
Epidemiology of Bovine Mastitis in Ethiopia
In Veterinary medicine, Mastitis is referred to an intramammary inflammatory reaction caused by physical or chemical agents but bacteria accounts for the majority of cause and the majority of cases are infectious. In contrast, some authors in human literature consider Mastitis as either an infectious or non-infectious disease .
The causative agent of bovine Mastitis could be physical or chemical agents but bacteria accounts for the majority of cause and the majority of cases are infectious. The disease has been reported by several authors on the prevalence and major causes of bovine Mastitis mostly in cross bred dairy cattle in different parts of the country [2,13,23,24]. Several of these studies have shown the occurrence of Mastitis causing bacteria, including, Staphylococcus and Streptococcus as dominant and pathogenic species [25-28]. The majority of isolates were Staphylococcus aureus (S. aureus), followed by Coagulase negative staphylococci (CNS) Streptococcus agalactiae (S. agalactiae), and the lowest isolation was Bacillus species (Table 1).
|Species isolated||Area of study||Prevalence %|
|Staphylococcus aureus||Asella, Holleta district, Addis Ababa, Aleta Wondo, Hawella Tula and Aleta Chuko, Sebeta, North Shewa Zone, Adigrat, West Arsi Zone, Lemu Bilbilo district (Arsi Zone)||9.42-83.47|
|Coagulase negative staphylococcus||West Arsi Zone, Addis Ababa, Holleta, Debrezeit, Wolayta Sodo, Bahir dar||3.9-51.9|
|Streptococcus agalactiae||West Arsi Zone, Sebeta, Addis Ababa, Adigrat, Holleta, Arsi Zone, Debrezeit, Doba district (West Hararghe zone), Bahir dar||3.73-25.3|
|Streptococcus dysgalaciae||Sebeta, Addis Ababa, Holleta, Doba district, Bahir dar||4.48-10.6|
|Klebsiella pneumonia||West Arsi Zone, Addis Ababa, Holleta, Sidamo Zone||3.3-10.7|
|staphylococcus intermidius||Selale, Lemu Bilbilo district, zone, Doba district, Asella, Sidamo Zone||6.6-23.19|
|E. coli||West Arsi Zone, Sebeta, Addis Ababa, Adigrat, Holleta, Arsi Zone, Debrezeit, Doba district, Tullo district (West Hararghe Zone) Wolayta Sodo, Asella,||0.75-28.4|
|P. aureuginosa||Sebeta, West Arsi Zone, Selale, North Shewa Zone, Holleta, Doba district, Asella, Addis Ababa||0.72-9.7|
|Corynebacterium bovis (C. bovis)||Sebeta, Addis Ababa, Selale, North Shewa Zone, Holleta, Doba district||1.45-7.04|
|Bacillus species||Sebeta, Addis Ababa, Holleta, Doba district, Sidamo Zone, Asella||0.8-5.71|
|Micrococcus species||Sebeta, Addis Ababa, Holleta, Selale||2.28-22.2|
Table 1: Bacterial isolated from bovine clinical and subclinical Mastitis in different part of Ethiopia.
Types of Mastitis
Clinical Mastitis can be defined as a farmer observed abnormality in the milk and/or the udder. Clinical Mastitis then, is an observable disease [29-39]. Cows are visibly sick, or the milk is visibly abnormal. The incidence of clinical Mastitis ranges from 0 to more than 200 cases per 1000 cows per year [40-42]. In most studies the median incidence is around 20-25 cases per 100 Cows per year. Clinical Mastitis occurs in all dairy herds. Even well-managed herds, as judged by somatic cell count level and a high level of milk production, may be suffering from a high incidence of clinical Mastitis. Clinical Mastitis is mostly caused by bacteria. The most important are S. aureus, E. coli, Klebsiella spp. and Streptococci (S. uberis, S. dysagalactiae). Clinical Mastitis is only the ‘tip of the iceberg’.
Subclinical Mastitis is by far the costlier disease in the majority of herds, and is often defined as the presence of a microorganism in combination with an elevated somatic cell count (SCC) of the milk. Various SCC cut-off points for the definition of subclinical Mastitis have been proposed. Most recent research seems to agree to a cut-off point at about 250,000 cells [43,44]. Several researchers have shown a log-linear relation between the somatic cell count of the milk and the milk production of the cow. The production decreases linearly with an increase in the log (SCC). A production loss of approximately 2 lbs. per natural log step above 5 is reported by several authors. The subclinical form of Mastitis in dairy cows is important because this form is highly prevalent than the clinical form and it usually precedes the clinical form, of long duration, difficult to detect, it reduces milk production and it adversely affects milk quality .
Prevalence Of Mastitis In Ethiopia
Over the last several years, a number of studies are available that describe the prevalence of bovine Mastitis in different parts of the country [14,26,35,37,46-51]. The prevalence reports were heterogeneous and were affected by several factors including locality, breed, management and diagnostic methods used. Accordingly, the prevalence for all types of Mastitis (i.e., without considering the classifications) ranged from 0.4% to 81.1%. Studies on prevalence of bovine Mastitis in Ethiopia were usually made through physical clinical examination of the udder in cases of clinical Mastitis and through the use of one of the cow side tests for sub clinical cases. The most commonly used cow side test in the publications reviewed was CMT, followed by indicator paper test [32,37,47-50,52,53]. In both clinical and subclinical cases, authors have used bacteriological methods for isolation and identification of the causative agents (Table 2).
|Area||Region||Diagnostic test used||No. of animal tested||No. positive animal||Subclinical Mastitis||Clinical Mastitis||Prevalence (95% CI)|
|Holleta||Addis Ababa||CMT||444||302||46.80%||21.20%||68% (63.7-72.3)|
|Kofale and Shashemene districts||Oromia||CMT, bacteriological examination||358||136||30.70%||7.30%||38% (32.9-43.1)|
|Jikawo Woreda of Nuer Zone||Gambella Region||CMT||121||73||48.76%||11.57%||60.33% (51.63-69.03)|
|Mekelle small holder’s Dairy farms||Tigray Region||CMT||351||221||54.40%||3.90%||62.9% (57.8-68)|
|Small Holder Lactating Dairy Farms in Hawassa||SNNP||Indicator paper test (PT)||183||65||30.60%||4.90%||35.5% (28.6-42.4)|
|Areka Woreda and Wolayta Zone||SNNP||bacteriology||384||203||43.50%||9.40%||52.9% (48.1-57.9)|
|Bahir Dar||Amhara||CMT, bacteriology||384||242||59.38%||3.65%||63.02% (58.3-67.7)|
|Eastern Amhara||Amhara||CMT, bacteriology||215||112||42.10%||3.80%||52% (45.3-58.7)|
|Hawassa||SNNP||CMT, bacteriology||529||331||59.20%||3.40%||62.6% (58.3-66.7)|
|Tullo district, West Hararghe||Oromia||CMT bacteriology||384||62||10.90%||5.20%||16.1% (12.4-19.8)|
|Addis Ababa||Addis Ababa||CMT, bacteriology||499||373||55.10%||19.60%||74.7% (71-78.4)|
|Sidama Zone||SNNP||CMT, bacteriology||96||43||42.70%||2.08%||44.79% (35-54.6)|
|Kombolcha||Amhara||California Mastitis test||150||84||46%||10%||56% (48.2-63.8)|
|Debrezeit||Oromia||CMT, bacteriology||300||18||5.30%||0.70%||0.4% (-0.3-1.1)|
|Hawassa||SNNP||Indicator paper test (PT), bacteriology||201||61||25.40%||5.00%||30.3% (24.1-36.5)|
|Wolayta Sodo||SNNP||CMT, bacteriology||386||134||21%||34.7% (30-39.4)|
|Doba, West Hararghe||Oromia||CMT, bacteriology||384||89||15.89%||23.18% (19-27.4)|
|Asella||Oromia||CMT, bacteriology||66||44||54.50%||12.10%||66.6% (55.2-78)|
|Lemu Bilbilo, Arsi Zone||Oromia||CMT, bacteriology||300||126||36.7%,||5.30%||42% (36.5-47.5)|
|Addis Ababa||Addis Ababa||CMT, bacteriology||331||172||36.86%,||15.41%||52.27% (47-57.6)|
|Holleta Agricultural Research Center||Oromia||CMT, bacteriology||90||73||73.30%||7.80%||81.1% (73.1-89.1)|
Table 2: Prevalence of Mastitis in different area of Ethiopia.
Results and Discussion
Different authors have different idea regarding the predisposing factors of Mastitis in Ethiopia but most of them do agree on considering breed, age, parity and lactation stages as important risk factors influencing the prevalence of bovine Mastitis. Additional risk factors considered by some investigators include: tick infestation of udder, sequence of milking, cleanliness of floor, husbandry system, and hygienic practices of the udder. During the review of studies conducted in Ethiopia, it was observed that different authors use different intervals for the categories of risk factors they included in their studies. That made it impossible to use intervals in presentation of the reviews. For this reason, author based tabulation was used to summarize different risk factors in the following sub-sections.
Studies conducted in Ethiopia generally show an increasing trend in the prevalence of Mastitis with increasing exotic blood levels (Table 3). Accordingly, the prevalence is the highest in pure breeds followed by crosses; and indigenous zebu being less frequently affected than others. The increase in prevalence in exotic breeds as opposed to local indigenous zebus could be the indigenous zebu are low in milk production and Higher yielding cows are more susceptible to Mastitis. There are several reasons that contribute to higher yielding cows more susceptible to Mastitis . For instance, Production level, production systems under which the animals are kept. The risks of clinical and subclinical Mastitis increase significantly with increasing age of cows (Table 4). The highest prevalence in older cows is because of their largest teats and more relaxed sphincter muscles, which increase the accessibility of infectious agent in the cows’ udder .
|Breed||No of animal examined||Prevalence|
|Cross (local × HF)||66||48.5%|
Table 3: Prevalence of Bovine clinical and subclinical Mastitis associated with different breeds.
|Age||No of animal examined||Prevalence %|
|Young Adult (3-5)||22||40.91|
|3-5 years (Young adult)||323||65.0|
|6-10 years (adult)||176||93.2|
|Older than 12 years||20||45|
Table 4: Prevalence of Bovine clinical and subclinical Mastitis associated with different age group.
Stage of lactation affect Mastitis prevalence significantly as a research conducted in Ethiopia implies (Table 5).
|Lactation stage||No of animal examined||Prevalence %|
|3 Week-4 Month||223||50.7|
|Early (<4 m)||37||56.76|
|Mid (5-7 m)||74||55.41|
|Late (>7 m)||10||80.00|
|≥ 7 Months||145||64.1|
|Early (<4 month)||133||87.2|
|Mid (5-7 Month)||132||65.9|
|Late (>8 Month)||234||73.1|
|Early (1-120 days||228||43.85|
|Middle (120-240 days||137||32.25|
|End (<240 days)||21||23.80|
|Greater than 9 months||81||3.7|
Table 5: Prevalence of Bovine clinical and subclinical Mastitis associated with different lactation stage.
Early stage and the period of involution (late stage) of the mammary glands were the most susceptible stages. This is possibly due to absence of dry cow therapy regime that is considered major factor contributing to high prevalence at early lactation .
The likelihood of Mastitis is higher in multiparous cows having four or more calvings compared with primiparous cows as studies conducted in Ethiopia shows (Table 6). This partly, might be associated with the position of udder in older cows. Thus, all of the older cows particularly those with four or more parity had pendulous udder and it has also stated that cows with the most pendulous quarters appear to be the most susceptible to mammary infections .
|Parity||No of animal examined||Prevalence%|
|≥ 4 Calves||203||88.2*|
|Greater than 6||16||56.25*|
*these category is highly significantly different (p-value<0.05)
**these categories are not significantly different (p-value>0.05)
Table 6: Prevalence of Bovine clinical and subclinical Mastitis associated with different parity.
Prevalence of Mastitis is significantly associated with milking hygienic practice. Cows at farms with poor milking hygiene standard are severely affected than those with good milking hygiene practices [13,14]. This might be due to absence of udder washing, milking of cows with common milkers’ and using of common udder cloths, which could be vectors of spread especially for contagious Mastitis. Again, the prevalence is also high in animal with udder or teat injury than those with no injury .
Prevalence of Mastitis at cow level is higher in those farms<4 years’ duration of farming, feed provision before milking and milking of clinical cows at any stage, poor drainage/slope for stable area which results accumulation of liquid such as urine and water used for cleaning of udders during milking, the liquid material mixed with the feces of the cows that led to dirty udder and teat there infection could enter [54- 56]. The occurrence of Mastitis based on body condition and history of previous Mastitis was also reported .
Diagnostic Method Used
Clinical examination of Mastitis
Clinical examination was conducted to determine prevalence of clinical Mastitis. Udder was examined for visible abnormalities, symmetry, size, consistency, presence of lesions and/or ticks. Clinical Mastitis was recognized by some pathology in udder, which is manifested by sign of inflammation like swelling, pain, redness and heat in case of acute Mastitis. Whereas, hardening of the udder, blockage of the teats, atrophy or fibrosis and abscess formation were manifested in chronic Mastitis. Acute Mastitis was also recognized by change in milk color, presence of flakes and clots .
California Mastitis test (CMT)
This California Mastitis test (CMT) a screening test for subclinical Mastitis. This test is preferred to be screening tests for subclinical Mastitis as they can be used easily, yielding rapid as well as satisfied result which was used by taking a small sample of milk (approximately ½ teaspoon) An equal amount of CMT reagent was added to the milk and the paddle rotated to mix the contents. After approximately 10 seconds, the score was read while continuing to rotate the paddle. Results were recorded as T (trace), 1, 2 or 3 based on the level of precipitation (coagulation) .
Surf field Mastitis test
The samples were subjected to surf test. For this purpose, 3% Surf solution was prepared by addition of three grams of commonly used detergent powder 3 gm surf (Surf Excel, Unilever, Pakistan) in 100 ml of distilled water. Milk samples and surf solution were then mixed in equal quantities in Petri dishes. The formation of gel depicted the positive samples .
Indicator paper test
This test technique is used as screening test to determine the prevalence of subclinical in animal. Milk sample were tested using the paper by adding milk sample to test paper and observing the color change of paper. Sample is negative if the yellow color of paper is not changed or shows a slight change in color. A change of color from yellow to green or bluish green was recorded as positive .
White side test
The principle of this test is based on the increased number of leukocyte in mastitic milk. Milk sample are placed on a clean dry glass slide and add a drop of 4% sodium hydroxide and mix with a glass rod. If the milk is from animal having Mastitis, it becomes thickened and flakes appear. While the negative milk sample remain the same [59,60].
For bacteriological examination milk sample collection is required. While taking sample from cow teats towards sample collection were sampled first and then the far side ones. The first 3 to 4 streams of milk were discarded as it may complicate the diagnosis. After collection, the sample is subject to bacterial culture and isolation within 7 to 10 days .
As described by [61-63] aseptic procedures for collecting quarter milk samples were followed. The time chosen for milk sample collection was before milking. Udders and especially teats were cleaned by antiseptic’s and water and dried before sample collection. Each positive CMT milk sample was collected separately to avoid confusion and cross contamination and was subject to laboratory for routine bacteriological investigation and cultured onto 10% sheep blood agar and MacConkey agar plates [62,64].
The suspected colonies could be identified morphologically, microscopically, biochemically and culture with fine bacterial growth were considered as positive and those with no visible growth taken as negative .
The milk sample was centrifuged and stained smear made from the deposit. A Gram stain was used routinely. The ZehilNeelson staining was performed for rare cases when bacteria such as M. bovis are suspected.
For the primary isolation and identification of Mastitis causing microorganisms, colony size, shape, color, pigmentation, hemolytic characteristic, gram’s reaction, oxidase, O-F tests were performed. After these colonies were sub cultured to different media, such as Mannitol salt agar, MacConkey agar (Oxiod, Hampshire, England), Edward’s medium (Oxiod Hampshire, England), Eosin methylene blue medium (EMB) (Oxiod, Hampshire, England) to get a pure culture and the secondary biochemical tests such as, coagulase test, urease test, IMVIC tests, sugar tests were done for bacterial species identification. The procedures for the identified pathogens were referred from.
Conclusion and Recommendations
Mastitis is still a major problem to farms and the review findings suggested that Mastitis is one of important disease of dairy cattle. Subclinical form is the most prevalent type of Mastitis in Ethiopia. Stage of lactation, parity, age, breed inadequate hygienic condition of dairy environment, and milking hygiene were the most important risk factor contributing to the prevalence of Mastitis. In cow diagnosis of Clinical Mastitis is based on the appearance of abnormally appearing milk while diagnosis of Subclinical infection is more problematic since the milk appears normal but usually has an elevated somatic cell count. Diagnosis of Subclinical Mastitis can be made in a variety of ways including direct measurement of the somatic cell count (SCC) level or indirectly by performing a California Mastitis test (CMT) on suspected quarters. Several causative agents of Mastitis were reported in Ethiopia. Staphylococcus aureus, Streptococcus agalactiae and E. coli were observed to be the major causes of Mastitis.
Having the above conclusions, the following recommendations are forwarded:
• Regular screening for early detection and treatment, follow up of chronic case, and control of subclinical Mastitis are recommended to alleviate the problem.
• Adequate research has to be made to figure out the prevalence at country level and take appropriate control measures.
• The sub clinical Mastitis which is highly prevalent and economically important should gain attention. In this regard awareness should be created on the importance of this type of Mastitis to farmers.
• Poor milking, environmental and personnel hygiene should be avoided in order to prevent cross contamination and increased chance of infection.
A detailed research regarding the prevalence and risk factors distribution of the disease should be made which enable effective control measures.
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Citation: Ismael A (2018) Epidemiology of Bovine Mastitis in Ethiopia. J Vet Med Health 2: 104.
Copyright: © 2018 Ismael A. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.