alexa Tendencies towards a C4 Leaf: Quantitative Studies on Leaf Anatomy of Selected C3 and C4 Grasses

ISSN: 2375-4338

Rice Research: Open Access

  • Research Article   
  • J Rice Res 2018, Vol 6(1): 188
  • DOI: 10.4172/2375-4338.1000188

Tendencies towards a C4 Leaf: Quantitative Studies on Leaf Anatomy of Selected C3 and C4 Grasses

Hiruni Nuwanthika Weerasooriya, Anil Jayasekera* and Iroja Caldera
Department of Plant Sciences, University of Colombo, Sri Lanka
*Corresponding Author: Anil Jayasekera, Department of Plant Sciences, Faculty of Science, University of Colombo, Sri Lanka, Tel: +94 77 8941187, Email: [email protected]

Received Date: Dec 19, 2017 / Accepted Date: Jan 12, 2018 / Published Date: Jan 15, 2018

Abstract

Grass family (Poaceae) is one of the largest families that represent monocots; containing species like Oryza sativa, Triticum aestivum, Zea mays, Eleusine coracana, Panicum miliaceum, Panicum sumatrense and Sorghum bicolor which are important to humans since they, directly or indirectly provide more than 3/4 of our food. C4 photosynthesis is a complex physiological adaptation, that is believed to have resulted from a series of anatomical and biochemical modifications to the ancestral C3 pathway. It is believed that C4 photosynthesis to have evolved in a stepwise manner nearly 22-24 times creating intermediates with different combinations of C4 -like components in grasses. C4 photosynthesis confers greater productivity than the C3 photosynthetic type in environments under high irradiance and high temperature. Accompanying higher photosynthetic rate C4 plants possess a vascular system in leaves with higher export rates which permit efficient translocation of photosynthates.

In the current study, selected 11 grass species were subjected to analysis of leaf vascular system in relation to their C3 and C4 photosynthesis. The leaf vascular network in both C3 and C4 grasses consist of LLV, SLV and TV that are connected to longitudinal veins. Analysis carried out using the mature leaves of selected C3 and C4 grasses have shown that there are significant differences in anatomical features such as venation, stomata and bundle sheaths. The comparison of DLLV, DSLV and DTV of C3 and C4 grasses demonstrate that C4 grasses have a denser vascular pattern compared to C3 species (respective distances of C4 grasses were 71.74 μm, 11.85 μm and 81.4 μm). It has been found that these reduced distances between veins have led to significant low stomatal density among C4 grasses. Further, characteristic large bundle sheath structure of C4 species has been clearly seen from significantly high values obtained for BSD, ISD and OSD (respectively 10.4 μm, 4.32 μm and 7.15 μm).

Furthermore, this screening has found Oryza nivara (a C3 species) having C4 like anatomical characters in leaf venation and bundle sheath structure. This species showed significantly low mean values for the distance between transverse veins (41.75 μm) resulting in significantly high total vein length per unit leaf area (81.77μm-1). The C4 tendency of its leaf anatomy was further supported by significantly large bundle sheaths, inner sheath and outer sheath distances (13.1 μm, 5.22 μm and 8.22 μm respectively).

At the end of the analysis we were able to demonstrate significant differences of anatomical characters like venation, stomata and bundle sheaths in C3 and C4 plants. In addition, observations suggest characteristic leaf anatomy that is compatible with C4 anatomy may have evolved in Oryza nivara resulting it to be considered as C3- C4 intermediate.

Keywords: C3-C4 intermediate; Grasses; Leaf vascular system; Longitudinal veins; Poaceae

Abbreviations

LLV: Large longitudinal veins; SLV: Small longitudinal veins; TV: Transverse veins; DLLV: Distance between large longitudinal veins; DSLV: Distance between small longitudinal veins; DTV: Distance between transverse veins; BSD: Bundle sheath distance; ISD: Inner sheath distance; OSD: Outer sheath distance; GPWG: Grass phylogeny working group; FAO: Food and Agricultural organization; PGRC: Plant Genetic Resource Center; RRDI: Rice research and Development Institute.

Introduction

Photosynthesis is a highly regulated, multistep process. It encompasses the harvest of solar energy, transfer of excitation energy, energy conversion, electron transfer from water to NADP+, ATP generation and synthesis of carbohydrates. Majority of plant species perform C3 photosynthesis [1] where CO2 is initially fixed by the enzyme Rubisco (ribulose-1, 5- bisphosphate carboxylase⁄oxygenase). However, Rubisco has the same affinity to react with CO2 as well as O2, which leads to photorespiration. It is known that photorespiration which consumes energy and releases previously fixed CO2 (causing a loss of 40% of photosynthates), is thought to be the driving force behind the evolution of C4 [2,3].

C4 leaves are characterized by Kranz-type anatomy, in which the vascular bundle is surrounded by bundle sheath (BS) cells and this tissue layer is further surrounded by radially arranged mesophyll cells. In contrast, C3 leaves have mesophyll cells that are well developed relative to the BS cells. However, these mesophyll cells of C3 plants contain only a few organelles [4]. In C4 photosynthesis, atmospheric CO2 is initially fixed in the mesophyll cells, then decarboxylation and refixation of CO2 occur in the BS cells [5]. Along with the changes in BS anatomy, some studies have reported that C4 leaves have a denser vascular system than C3 leaves [6]. This is clearly seen in leaves of grasses, which possess parallel venation. Leaves of C3 and C4 species usually express a distinct difference in the interveinal distances leading to the denser vascular system of C4 species. Denser vascular system may also be needed for the expression of photosynthetic enzymes in the mesophyll and bundle sheath cells [7]. C4 plants exhibit higher photosynthetic rates than C3 plants even under high irradiance and at high temperatures [8].

Evolution of C4 plants from C3 has been accompanied by several anatomical and biochemical modifications of leaves. A change in the vein density has evidently occurred during the evolution from C3 to C4. Veins of grass leaves show a hierarchical order and they are differentiated into large, small and transverse veins. Further, they show a clear division of labour. The large longitudinal veins (LLV) serve primarily in longitudinal transport of photosynthate. The small longitudinal veins (SLV) serve primarily in collecting photosynthate from nearby photosynthetic cells whereas transverse veins (TV) connect the longitudinal veins and play an important role in the lateral transport of photosynthate from the small to the large longitudinal veins [9]. In studying the vascular architecture of the leaf, localization of the veins and their distinct functions are important. Most of the previous studies on C3 and C4 grass leaves have mainly focused only on large longitudinal veins [6,10-12], while studies of Ueno et al. [4] and Oguro et al. [13] had given equal emphasis to studying transverse vein densities as well.

C4 origins are not randomly distributed across the plant tree of life. Most large (species rich) clades of plants completely lack C4 taxa where as others contain an astonishingly high number of C3 to C4 transitions. This is observed predominantly in grasses, in which C4 photosynthesis has evolved independently 22-24 times [13]. Repeated origins of C3 and C4 photosynthesis in grasses occurred only in PACMAD clade (Panicoideae, Arundinoideae, Chloridoideae, Micrairoideae, Aristidoideae and Danthonioideae) [14,15].

Previous studies have shown that stomatal characters vary predictably between C3 and C4 species, clearly attributed to the functional convergence based on photosynthetic pathway [16,17]. Since C4 species have high photosynthetic rates even at low intercellular CO2 concentrations, they maintain lower stomatal conductance rates than C3 species to reduce their transpiration rate and further increase their water-use efficiency (the ratio of photosynthesis to transpiration) [18]. Generally, C4 plants have achieved lower stomatal conductance rates by producing smaller stomata for a given stomatal density. It has been found that some C4 lines have reduced stomatal density, while others have a reduced stomatal aperture [2].

To the best of our knowledge, there have been very few studies conducted focusing on leaf anatomy in relation to the photosynthetic mechanism in grass species in Sri Lanka. Therefore, this study was carried out with the main objective of studying differences in the venation, bundle sheath anatomy and stomatal characters of selected C3 and C4 grass species of Sri Lanka.

Materials and Methods

Plant materials

Eleven representative species of both C3 and C4 photosynthetic groups were used for the study (Table 1). C4 species were further divided into two subcategories as NADP-ME and NAD-ME, according to their decarboxylation mechanisms.

Photosynthetic mechanism                           Plant species
C3 Oryza sativa (BG 357, BG 403, BG 94-1), Oryza nivara, Isachne globosa
C4  
NADP-ME Setaria italica, Sorghum bicolor, Zea mays
NAD-ME Panicum sumatrense, Panicum miliaceum, Eleusine coracana

Table 1: Plant species used in the study.

Experimental design

Seeds of selected plant species were obtained from PGRC (Plant Genetic Resource Centre, Sri Lanka) and RRDI (Rice Research and Development Institute, Bathalagoda, Sri Lanka). Seeds were sown in pots filled with a mixture of sand, compost and soil (1:1:2 volume ratios). They were allowed to germinate in a glass house. Temperature was recorded as 30.25°C ± 1.7°C and relative humidity as 72% ± 2.16% throughout the experimental period. Plants were watered daily with adequate amounts.

Sampling of leaves and fixation

Fully expanded leaves were used for the study [14]. Leaves were tagged and sampled at twenty days. Leaf blades were cut and immediately fixed in a mixture of formaldehyde, acetic acid and ethanol in water (1:1:18 volume ratios).

Venation studies

Leaf clearing: Cleared leaf blades were prepared according to the method described by Ueno [19]. The middle portions of fixed leaves were boiled in 70% ethanol for about 10-20 minutes. After washing in distilled water several times, they were transferred to boiling 85% lactic acid for 20 min, and then stored in chloral hydrate-saturated in ethanol before analysis. Slides were prepared and observed (without staining) under a light microscope.

As a modified methodology some leaves were cleared by soaking in a solution of formaldehyde: propionic acid: ethanol (1:2:18) over night. Slides prepared by this method were stained using safranin and observed under light microscope.

Quantitative data of leaf vascular systems: Leaf veins were identified as three types: large longitudinal veins (LLV), small longitudinal veins (SLV) and transverse veins (TV) [19,20]. The two types of longitudinal veins were distinguished by diameter under the light microscope. The distances between SLV, LLV and TV were represented by means of 30 measurements of middle portions of five leaf blades taken from five plants. The distance between longitudinal veins was measured between the centers of adjacent veins. The transverse veins are usually curved, unlike the parallel longitudinal veins. For the distance between TV, therefore, the mean of the minimum and maximum distances between adjacent transverse veins running between a pair of longitudinal veins was calculated. The distance between SLV was multiplied by that between TV for each species to obtain areolar area, which represents the minimum area of photosynthetic tissue surrounded by veins. Total vein length was measured using the lengths of large, small and transverse veins in one field.

Stomatal studies

Stomatal imprints were obtained using the method described by [21]. Stomatal index (Stomatal density/(Stomatal density+Epidermal cell density) and stomatal density were calculated using nail polish imprints obtained from abaxial and adaxial surfaces of leaves. These measurements were represented by means of thirty measurements of five leaf blades taken from five different plants.

Bundle sheath observations

Hand sectioning was done to make observations on vascular bundles. Bundle sheath distance, outer sheath distance and inner sheath distances were obtained from the leaf cross sectioning. Distance measurements were calculated by obtaining average of three diameters. Bundle sheath, outer and inner sheath areas were calculated using the distance measurements (Figure 1). Demonstration of these areas was carried out following Christin et al. [14]. Means of these measurements were represented by fifteen measurements of five leaf blades taken from five different plants.

rice-research-Outer-sheath-distance

Figure 1: Representation of measured distances of vascular bundle (modified diagram from Christin et al. [14] (BSD- Bundle sheath distance, OSD- Outer sheath distance, ISD- Inner sheath distance).

Statistical analysis

Differences in the mean values generated for each species were tested at 0.05 significance for each species between the C4 and C3 groups using one- way analysis of variance (ANOVA) with Tukey’s honesty significance difference test (statistical software SPSS version 16.0).

Principal component analysis

Principle Component analysis was carried out with PAST (Paleontological statistics software package for education and data analysis) version 2.17C.

Results

The leaf vascular network in both C3 and C4 grasses consist of LLV, SLV and TV that are connected to longitudinal veins (Figure 2). The comparison of distances measured between large longitudinal veins, small longitudinal veins and transverse veins of C3 and C4 grasses demonstrate that C4 grasses have a denser vascular pattern compared to C3 species. Comparison of mean values obtained for distances measured between large longitudinal veins, small longitudinal veins, transverse veins for the C3 and C4 species reveal that C3 species have a 1.3, 2.44 and 1.4 times significantly higher value than that of C4 grasses. In addition, compared to C3 species, C4 species recorded a 1.03 and 1.47 times significantly higher mean value for areolar area (minimum photosynthetic area surrounded by veins) and total vein length per unit leaf area respectively (Table 2).

rice-research-Paradermal-view

Figure 2: Paradermal view of the cleared leaf blade. A-Setaria italica (C4) B-Panicum miliaceum (C4) C-Oryza nivara (C3) D-Oryza sativa (C3), Scale bars=10 μm). LLV - Large longitudial veins; SLV - Small longitudinal veins; TV - Transverse veins.

  DLLV(µm) DSLV(µm) DTV(µm) Areolar area (µm2) Total vein length per unit leaf area (µm-1)
C3 94.26 ± 3.04A 28.97 ± A 112.5 ± 6.14A 1.89 ± 0.089A 73.85 ± 0.8A
Oryza sativa          
BG  403 77.29a 15.09c 156.8a 1.674a 77.01a
BG 357 71.29a 14.86a 164.1a 1.56a 73.08a
BG 94-1 79.67a 15.29a 157.85a 1.462a 70.53a
Oryza nivara 77.38a 14.82a 41.75b 1.42a 81.77b
Isachne globosa 165.66c 84.79d 42b 3.54c 66.87c
C4 71.74 ± 2.68B 11.85 ± 0.16B 81.4 ± 1.9B 1.95 ± 0.099B 108.78 ± 1.66B
NADP-ME 76.09 ± 1.99A' 11.71 ± 0.2A' 79.43 ± 2.91A' 2.6 ± 0.15A' 110.29 ± 1.83A'
 Setaria italica 64.3d 9.62a 91.8d 1.2b 130.21d
Sorghum bicolor 77.64b 10.65b 115.3c 1.56d 117.34e
Zea mays 86.34b 14.86e 31.2b 4.99e 83.33b
NAD-ME 54.07 ± 1.92B' 11.99 ± 0.26B' 83.27 ± 0.87B' 1.31 ± 0.02B' 107.21 ± 2.88B'
Panicum miliaceum 44.33e 11.23b 83.36e 1.25b 118.56f
Panicum sumatrense 44.58f 11.33b 86.8f 1.3b 126.97g
Eleusine coracana 73.29b 13.42f 79.7g 1.37b 76.27h

Within columns, values followed by the same case letter are not significantly different according to Tukey’s honesty significant difference (HSD) test (P<0.05).
Bold-face values indicate significant difference among the mean values of species representing genus Oryza.

Table 2: Comparison of vein parameters among C3 and C4 species.

There have been several studies focused on the leaf vasculature of C3 and C4 grasses [4,12,14]. In a extensive study on leaf vasculature system in C3 and C4 grasses [4] reported a trend similar to the trend recorded in the present study where a 1.1, 2.2 and 1.9 times higher value for distance between large, small and transverse veins of C4 species were measured compared to C3 plants. Furthermore, Ueno et al. [4] had reported a 4.3 and 2.1 times higher value for areolar area and total vein length per unit leaf area in C4 plants.

All the other vein parameters except distance between TV and total vein length per unit leaf area were similar among O. nivara and the other varieties of O. sativa . However, values obtained for these two parameters of O. nivara and Zea mays (C4 grass) were not significantly different. Thus, the present study has been figured out for some vein parameters there is similar tendency between O. nivara and C4 species where it was not so for the species among same genus Oryza . The results obtained have further shown that the significantly low mean value of distance between transverse veins (41.75 μm) of O. nivara has led to a significantly high total vein length per unit leaf area of 81.77 μm-1.

The C4 photosynthetic carbon cycle is an elaborated addition to the C3 photosynthetic pathway. In C4 plants the CO2 concentration mechanism is achieved by division of labor between two distinct, specialized leaf cell types, the mesophyll and the bundle sheath cells [22]. Thus, bundle sheaths play a major role in evolution of C3 pathway to C4. Christin et al. [14], in a study of quantitative bundle sheath anatomy has shown that C4 evolvability strongly increases when the proportion of vascular bundle sheath tissue is higher than 15%, which results from a combination of short distance between bundle sheaths and large bundle sheath cells. Indicating large bundle sheath structure of C4 species, the present study has found 15.9 percent increment of bundle sheath thickness in C4 plants (Table 3), a similar trend that has been recorded in previous studies as well [12,14,17].

  Bundle sheath distance (µm) Outer sheath distance (µm) Inner sheath distance (µm) Outer sheath area (µm2) Inner sheath area (µm2)
C3 8.9 ± 0.03A 6.21 ± 1.02A 3.7 ± 0.11A 29.6 ± 2.92A 10.87 ± 1.11A
Oryza sativa          
BG 403 6.13a 5.2a 2.2a 19.63a 3.14a
BG 357 6.23a 5.1a 2.5a 19.63a 4.9a
BG 94-1 8.2a 5.3a 3.4c 19.63a 7.07c
 Oryza nivara 13.1b 8.22b 5.22b 50.24b 19.63b
Isachne globosa 11.2b 7.23b 5.12b 38.47b 19.63b
C4 10.4 ± 0.01B 7.15 ± 0.03B 4.32 ± 0.72B 44.7 ± 1.12B 12.95 ± 2.12B
NADP-ME 12.1 ± 1.21A' 8.76 ± 0.72A' 4.4 ± 0.23A' 65.8 ± 2.01A' 15.2 ± 3.02A'
Setaria italica 9.84c 5.78a 3.09a 26.41a 7.64a
Sorghum bicolor 12.0d 8.1b 4.3c 50.24b 12.56c
Zea mays 14.3e 12.41d 5.7b 120.7e 25.5b
NAD-ME 8.81 ± 0.89B' 5.54 ± 0.71B' 3.9 ± 0.51B' 23.6 ± 1.51B' 10.7 ± 2.81B'
Panicum miliaceum 8.1c 5.22a 4.2c 19.625a 12.56c
Panicum sumatrense 8.21a 4.3c 3.1a 12.56c 7.07a
Eleusine coracana 10.11b 7.1b 4.41c 38.47d 12.56c

Within columns, values followed by the same case letter are not significantly different according to Tukey’s honesty significant difference (HSD) test (P<0.05). Bold-face values indicate significant difference among the mean values of species representing genus Oryza

Table 3: Comparison of bundle sheath distances and area among C3 and C4 species.

Among the few representatives of genus Oryza , O. nivara showed a significantly high value for the Oryza nivara and other Oryza varieties, a few C4 species had shown no significant difference for the respective parameters (Table 3). Furthermore, a similar trend has been observed in mean values of outer sheath and inner sheath areas. Bundle sheath distance (13.1 μm), outer sheath distance (8.22 μm) and inner sheath distance (5.22 μm). While mean values of bundle sheath, outer sheath and inner sheath distances were significantly different.

Stomatal characters vary predictably between C3 and C4 species attributing to their photosynthetic pathway [17]. Among the C3 and C4 representatives, C4 plants have achieved low stomatal conductance by low density of smaller stomata [2]. The present research has clearly demonstrated a significant difference in stomatal density and index between C3 and C4 species (Table 4).

Species name Stomatal index Stomatal density (µm-2)
Oryza sativa    
     BG  403 0.0203a 1100a
     BG 357 0.0302a 1000a
     BG 94-1 0.023a 1210a
Oryza nivara 0.0404e 1150a
Panicum miliaceum 0.1521b 310d
Panicum sumatrense 0.1727c 260c
Sorghum bicolor 0.1353b 270c
Eleusine coracana 0.1432b 100e
Zea mays 0.1493b 150b
Isachne globosa 0.1016d 140b
Setaria italica 0.161c 160b

Within columns, values followed by the same case letters are not significantly different according to Tukey’s honesty significant difference (HSD) test (P<0.05).
Bold-face values indicate significant difference among the mean values of species representing genus Oryza.

Table 4: Comparison of stomatal index and stomatal density of C3 and C4 grasses.

Among the C3 and C4 species, genus Oryza had the highest density of stomata (1000-1210 μm-2). While there have been several studies that explain the theory behind this phenomenon, Kawamitsu et al. [23] has reported rice plants growing in wet places may have high evaporative demand (due to high density of stomata) in order to lower the surface temperature of the leaf on a hot dry day. On the other hand this may lead to intense transpiration from the leaf blade resulting in water stress conditions in the plant. It has been reported that in some paddy fields rice plants are subjected to water stress at mid-day even with adequate supply of water due to high density of stomata [4].

Furthermore, it has been identified that high density of veins and the shortest distance between them have resulted in low stomatal density in C4 plants (Figure 3).

rice-research-leaf-epidermis

Figure 3: Schematic representation of stomatal spread on a C3 and C4 leaf epidermis; A - C4 leaf epidermis (dense venation low stomatal density); B – C3 leaf epidermis (less number of veins and high stomatal density).

Unlike the bundle sheath anatomy and venation, there were no records for similar stomatal density values among O. nivara and C4 species. Thus, it has been found that these significant changes of Oryza nivara leaf anatomy have only been due to its bundle sheath anatomy and venation.

From the principal component analysis (PCA) a clear association of C3 and C4 species (Figure 4) is seen. Most weighted characters that had contributed to the main principle components were distance between large longitudinal veins, distance between small longitudinal veins and outer sheath distance. Reflecting the differences of traits associated to the leaf anatomy, all the varieties of Oryza sativa (BG 403, BG 357 and BG 94-1) clustered together while Oryza nivara has clustered with the other C4 species.

rice-research-Principle-Component

Figure 4: PCA scatter diagram produced by plotting the first Principle Component (PC) against the second PC.

In the classification of grasses, genus Oryza is placed within the BEP clade, one of the major clades that contain only C3 origins [24]. During the last year, percentage increment of annual rice yield has been recorded as 0.9%. Further, it is expected that the world rice inventories might drop by 3% in 2017, which if confirmed, would be the second consecutive season of declines [25]. Thus, there is a strong evidence that yield potential in rice (Oryza sativa L.) is becoming limited by ‘source’ capacity (photosynthetic capacity or efficiency), and hence the ability to further increase the yield in modern varieties [26]. Thus, among different approaches in developing enhanced photosynthetic capacity of rice, introducing C4 pathway has become one of the focal points and challenges [3]. Thus, screening selected C3 and C4 grasses, in the context of understanding developed characteristic features of C4 species is important to lay a good foundation in manipulating the anatomy and the physiology of the rice leaf.

The current study has revealed that anatomical features like venation and bundle sheaths of Oryza nivara have contributed to its improved leaf anatomy; so called “C4 tendency”. This, as mentioned before can be considered as one of the important facts that might open up new insights in to engineering the rice leaf. Oryza nivara is one of the common and widely distributed wild Oryza species found in Sri Lanka. It is generally found in shallow water and swampy areas. These plants have shown resistance to different biotypes of brown plant hoppers, green leaf hopper, grassy stunt virus and blast and partial resistance to stem rot [27]. The study of Rizal et al. [28] has reported several accessions of Oryza nivara with low CO2 compensation points, a developed physiological tendency compared with the rest of C3 species. Further, several studies have focused on Oryza nivara where the plant been used in breeding strategies to enhance the yield of Oryza sativa [29,30]. But, no records have been found related to the anatomical traits of this species. Thus, this paper mainly emphasizes the developed leaf anatomy of this species showing a potential “C4- ness”.

Conclusion

Based on the results of this study, it can be concluded that C4 grasses have denser vein system, large bundle sheaths and low stomatal density compared to C3 grasses that were used in this study. In addition, observations suggest that some characteristic features of C4 leaf anatomy may have evolved in Oryza nivara resulting it to be considered as C3-C4 intermediate. Furthermore, it is also suggest that possession of C4 leaf anatomical features in Oryza nivara could be used as a source of germplasm to bring about a change in the leaf anatomy of Oryza sativa that would contribute towards“C4-ness” and subsequently enhancing the photosynthetic efficiency of rice.

Competing Interests

The authors declare that they have no competing interests.

Authors’ Contributions

Conceived and designed the experiments: AJ, IC, HNW. Performed the experiments: HNW. Analyzed the data: HNW. Wrote or proofread the paper: AJ, IC, HNW. All authors read and approved the final manuscript.

Acknowledgements

We are grateful to Dr. A. Bentota, Director General, RRDI, Bathalagoda, Sri Lanka, Dr. K. Hettiarachchi, Director, Seed Certification & Plant Protection Centre, Gannoruwa, Sri Lanka and Prof. B. Marambe for their support given.

References

Citation: Weerasooriya HA, Jayasekera A, Caldera I (2018) Tendencies towards a C4 Leaf: Quantitative Studies on Leaf Anatomy of Selected C3 and C4 Grasses. J Rice Res 6: 188. Doi: 10.4172/2375-4338.1000188

Copyright: © 2018 Weerasooriya HN, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Select your language of interest to view the total content in your interested language

Post Your Comment Citation
Share This Article
Relevant Topics
Article Usage
  • Total views: 425
  • [From(publication date): 0-2018 - Jul 18, 2018]
  • Breakdown by view type
  • HTML page views: 383
  • PDF downloads: 42

Post your comment

captcha   Reload  Can't read the image? click here to refresh
Leave Your Message 24x7