alexa Detection of Biological Environments for Endometrial Stromal and Mesenchymal Stem Cells Growth through a Quartz Crystal Microbalance Based Biosensor | Abstract
ISSN: 2090-4967

Biosensors Journal
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Research Article

Detection of Biological Environments for Endometrial Stromal and Mesenchymal Stem Cells Growth through a Quartz Crystal Microbalance Based Biosensor

Karekin D Esmeryan*

Georgi Nadjakov Institute of Solid State Physics, Bulgaria

Corresponding Author:
Esmeryan KD
Georgi Nadjakov Institute of Solid State Physics
72, Tzarigradsko Chaussee Blvd
1784 Sofia, Bulgaria
Tel: 9014170809
E-mail: [email protected]abv.bg

Received Date: May 22, 2015 Accepted Date: August 18, 2015 Published Date: August 21, 2015

Citation: Esmeryan KD (2015) Detection of Biological Environments for Endometrial Stromal and Mesenchymal Stem Cells Growth through a Quartz Crystal Microbalance Based Biosensor. Biosens J 4:120. doi:10.4172/2090-4967.1000120

Copyright: © 2015 Esmeryan KD. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The sensor response of 5 MHz gold electrode coated quartz crystal microbalances (QCMs) towards various environments for human endometrial stromal and mesenchymal stem cells growth is reported. The deposition of endometrial stromal cells onto sensor surface causes mass loading dependent resonance frequency downshift of 1.5 kHz and a corresponding increase in the dynamic resistance of 403 Ω. Furthermore, the QCMs demonstrate ability to detect negligible changes in the viscosity/density product of different biological environments. In addition, the sensors show reproducible sensor characteristics with maximum deviations from measurement to measurement within 50 Hz. The observed deviations are lower compared to the sensor signal, thereby confirming that the QCMs could be used as in situ detectors of various biological compounds or interactions, closely related to the in vitro fertilization analysis. These experiments open a possibility for integration of wide range in vitro investigations on lab-on-a-chip devices.

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