Development and Validation of a High Performance Liquid Chromatography Method for Determination of Telmisartan in Rabbit Plasma and its Application to a Pharmacokinetic Study
Prasad S. Virkar*, Satish G. Pingale and Kiran V. Mangaonkar
Department of Chemistry, Mithibai College of Arts, Chauhan Institute of Science and Amrutben Jivanlal College of Commerce and Economics, Vile Parle (W), Mumbai 400056, India
- *Corresponding Author:
- Prasad S. Virkar
Department of Chemistry
Mithibai College of Arts
Chauhan Institute of Science and Amrutben
Jivanlal College of Commerce and Economics
Vile Parle (W), Mumbai 400056, India
E-mail: [email protected]
Received date: March 03, 2012; Accepted date: May 26, 2012; Published date: May 30, 2012
Citation: Virkar PS, Pingale SG, Mangaonkar KV (2012) Development and Validation of a High Performance Liquid Chromatography Method for Determination of Telmisartan in Rabbit Plasma and its Application to a Pharmacokinetic Study. J Anal Bioanal Tech 3:133. doi: 10.4172/2155-9872.1000133
Copyright: © 2012 Virkar PS et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
A simple and sensitive High-Performance Liquid Chromatography (HPLC) method with fluorescence detection for quantitation of telmisartan in rabbit plasma was developed and validated. Separation of telmisartan from plasma components was achieved on a Chromolith RP18e column (100 x 4.6 mm 5 μ). Losartan was used as an internal standard. A mobile phase consisting of 50 mM sodium phosphate buffer and acetonitrile in the volume ratio of 65:35 v/v was delivered at a flow rate of 3.5 mL/min. A simple and rapid sample preparation involved solid phase extraction using Varian Bond Elute C-18 cartridge. The linearity range of the proposed method was 4 to 375 ng/mL. The intra-day and inter-day coefficients of variation obtained for telmisartan were less than 4.90% and relative error was less than 9.08%. The overall recoveries for telmisartan and losartan were 101.7% and 100.0%, respectively. This validated method was used successfully for analysis of plasma samples from a pharmacokinetic study.