alexa Development and Validation of a New HPLC Method for In-vitro Studies of Haloperidol in Solid Lipid Nanoparticles
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Development and Validation of a New HPLC Method for In-vitro Studies of Haloperidol in Solid Lipid Nanoparticles

Mohd Yasir1,2*, Udai Vir Singh Sara3, Iti Som2 and Lubhan Singh4

1Department of Pharmacy, Uttarakhand Technical University, Dehradun-248007, Uttarakhand, India

2Department of Pharmaceutics, I.T.S Pharmacy College, Delhi- Meerut Highway, Muradnagar, Ghaziabad-201206 (UP), India

3Department of Pharmaceutics, Dr. M C Saxena College of Pharmacy, Lucknow- 226101, (UP), India

4Ram-Eesh Institute of Vocational and Technical Education, Greater Noida, Gautam Budh Nagar-201310, Uttar Pradesh, India

*Corresponding Author:
Mohd Yasir
Department of Pharmaceutics, I.T.S Pharmacy College
Delhi- Meerut Highway, Muradnagar, Ghaziabad-201206 (UP), India
Tel: 91-9761131206
Fax: (01232) 225380
E-mail: [email protected]

Received Date: October 31, 2016; Accepted Date: November 16, 2016; Published Date: November 21, 2016

Citation: Yasir M, Sara UVS, Som I, Singh L (2016) Development and Validation of a New HPLC Method for in-vitro Studies of Haloperidol in Solid Lipid Nanoparticles. J Anal Bioanal Tech 7:339. doi: 10.4172/2155-9872.1000339

Copyright: © 2016 Yasir M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

 

Abstract

A simple and sensitive HPLC method was developed and validated for the study of in-vitro drug release from haloperidol loaded solid lipid nanoparticles (SLNs). The method was also used for the analysis of drug for detection of shelf life of developed SLNs. Chromatogram separation was achieved using C18 column as stationary phase. The mobile phase consisted of 100 mM/L potassium dihydrogen phosphate–acetonitrile-TEA (10:90:0.1, v/v/v) and the pH was adjusted with o-phosphoric acid to 3.5. Flow rate of mobile phase was 2 ml/minute and eluents were monitored at 230 nm using UV/VIS detector. The method was validated for linearity, precision, accuracy, reproducibility, limit of detection (LOD) and limit of quantification (LOQ). Linearity for haloperidol was in the range of 1-60 μg/mL. The value of LOD and LOQ was found to be 0.045 and 0.135 μg/ml respectively. The drug loaded SLNs showed sustained drug release with maximum value of 95.52 ± 5.21% in 24 h. The shelf life of SLNs formulation was found to be 2.31 years at 4°C.

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