alexa

GET THE APP

Dersleri yüzünden oldukça stresli bir ruh haline sikiş hikayeleri bürünüp özel matematik dersinden önce rahatlayabilmek için amatör pornolar kendisini yatak odasına kapatan genç adam telefonundan porno resimleri açtığı porno filmini keyifle seyir ederek yatağını mobil porno okşar ruh dinlendirici olduğunu iddia ettikleri özel sex resim bir masaj salonunda çalışan genç masör hem sağlık hem de huzur sikiş için gelip masaj yaptıracak olan kadını gördüğünde porn nutku tutulur tüm gün boyu seksi lezbiyenleri sikiş dikizleyerek onları en savunmasız anlarında fotoğraflayan azılı erkek lavaboya geçerek fotoğraflara bakıp koca yarağını keyifle okşamaya başlar
Effect of the Storage Temperatures on BRL-encapsulated-alginate-PLO-alginate (APornA) Microcapsules | OMICS International | Abstract
ISSN: 1662-100X

Journal of Biomimetics Biomaterials and Tissue Engineering
Open Access

Our Group organises 3000+ Global Conferenceseries Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ Open Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.

Open Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers

This Readership is 10 times more when compared to other Subscription Journals (Source: Google Analytics)

Research Article

Effect of the Storage Temperatures on BRL-encapsulated-alginate-PLO-alginate (APornA) Microcapsules

Yuan-Gang Liu1,2*, Yan Bai1, Shi-Bin Wang1,2*, Ai-Zheng Chen1,2 and Wen-Guo Wu1,2

1College of Chemical Engineering, Huaqiao University, Xiamen 361021, China

2Institute of Biomaterials and Tissue Engineering, Huaqiao University, Xiamen 361021, China

Corresponding Authors:
Yuan-Gang Liu
College of Chemical Engineering
Huaqiao University, Xiamen 361021, China
Tel: +86 592 6162326
Fax: +86 592 6162326
E-mail: [email protected]
 
Shi-Bin Wang
Institute of Biomaterials and Tissue Engineering
Huaqiao University, Xiamen 361021, China
E-mail: [email protected]

Received date July 17, 2013; Accepted date August 23, 2013; Published date August 25, 2013

Citation: Yuan-Gang L, Bai Y, Shi-Bin W, Ai-Zheng C, Wen-Guo W (2013) Effect of the Storage Temperatures on BRL-encapsulated-alginate-PLO-alginate (APornA) Microcapsules. J Biomim Biomater Tissue Eng 18:110. doi: 10.4172/1662-100X.1000110

Copyright: © 2013 Yuan-Gang Liu, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

To obtain long-term storage of cell-encapsulated alginate-PLO-alginate (APornA) microcapsules, the influences of storage temperatures on the microcapsule morphology, cell viability and metabolic activities were investigated. The results showed that the microcapsules exhibited the original morphological characteristics with smooth surface, good sphericity and overall integrity after 4 weeks preserved in liquid N2. 4°C and -20°C were presented to be the harsh conditions for cell propagation and the secretion of metabolic products compared to -80°C and -196°C. It can be concluded that lower temperature is beneficial for the cell recovery. These results supplied supplementary data for poly- L-ornithine (PLO) as a potential coating material in the future cell microencapsulated applications.

Keywords

Top