alexa Efficiency of In Vitro Regeneration is Dependent on the
ISSN: 2329-8863

Advances in Crop Science and Technology
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Research Article

Efficiency of In Vitro Regeneration is Dependent on the Genotype and Size of Explant in Tef [Eragrostis tef (Zucc.) Trotter]

Zerihun Tadele*, Sonia Plaza-Wüthrich and Regula Blösch
Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland
Corresponding Author : Zerihun Tadele
Institute of Plant Sciences
University of Bern, Altenbergrain 21
3013 Bern, Switzerland
Tel: +41 31 631 4956
Fax: +41 31 631 4942
E-mail: [email protected]
Received July 01, 2015; Accepted July 29, 2015; Published August 04, 2015
Citation: Tadele Z, Wüthrich SP, Blösch R (2015) Efficiency of In Vitro Regeneration is Dependent on the Genotype and Size of Explant in Tef [Eragrostis tef (Zucc.) Trotter]. Adv Crop Sci Tech 3:179. doi:10.4172/2329-8863.1000179
Copyright: © 2015 Tadele Z, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Tef [Eragrostis tef (Zucc.) Trotter] is the major cereal crop in the Horn of Africa particularly in Ethiopia where it is staple food for about 50 million people. Its resilience to extreme environmental conditions and high in nutrition makes tef the preferred crop among both farmers and consumers. The efficiency of in vitro regeneration plays significant role in the improvement of crops. We investigated the efficiency of regeneration in 18 tef genotypes (15 landraces and three improved varieties) using three sizes of immature embryos (small, intermediate and large) as an explant. In vitro regeneration was significantly affected by the genotype and the size of the immature embryo used as a donor. Intermediate-size immature embryos which were 101-350 µm long led to the highest percentage of regeneration. Interestingly, the three improved varieties presented very low regeneration efficiencies whereas the landrace Manyi resulted in consistently superior percentage of in vitro regeneration from all three sizes of explants. The findings of this work provide useful insight into the tef germplasm amenable for the regeneration technique which has direct application in techniques such as transformation. It also signifies the importance of using tef landraces instead of improved varieties for in vitro regeneration.


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