Evaluating Effect of Di-potassium Hydrogen Phosphate (K2HPO4) on Accumulation of Some Secondary Metabolites in Spirulina cyst
- *Corresponding Author:
- Nematzadeh GH
Professor of Plant Genetic
Agricultural University of Sari, Iran
E-mail: [email protected]
Received Date: December 22, 2014; Accepted Date: January 03, 2015; Published Date: January 10, 2015
Citation: Nematzadeh GH, Fathalipour K, Pishdad, Azad HN, Esmaeili A (2015) Evaluating Effect of Di-potassium Hydrogen Phosphate (K2HPO4) on Accumulation of Some Secondary Metabolites in Spirulina cyst. J Ecosys Ecograph 5:155. doi:10.4172/2157-7625.1000155
Copyright: © 2015 Nematzadeh GH, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Marine microalgae constitute natural sources of carotenoids used in a variety of drugs, and pharmaceutical, food and cosmetic applications. In this study, inducible effects of K2HPO4 on the accumulation of some apo carotenoids including chlorophyll-a and b, beta carotene, astaxanthin, lycopene and pheophytin were explored using a completely randomized design with five treatments and three replications. Cellular extracts were carried out with dimethyl sulfoxide solvent. A Duncan’s test was performed on data obtained through spectrophotometric absorption of studied metabolites. Our results showed a significant difference among the treatments. The metabolites content in cellular extracts at the stationary phase of algae were estimated at the highest level of 4.5 and 3.58 mg/g for chlorophyll-a, and beta carotene, respectively, in the fifth treatment level. Astaxanthin and lycopene were 1.505 and 2.84 mg/g, respectively, in the second treatment. There was no significant difference between the chlorophyll-b and pheophytin pigments when compared to the control.