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Ex Vitro Rooting of Sugarcane (Saccharum officinarum L.) Plantlets Derived from Tissue Culture | OMICS International | Abstract
ISSN: 2329-8863

Advances in Crop Science and Technology
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Research Article

Ex Vitro Rooting of Sugarcane (Saccharum officinarum L.) Plantlets Derived from Tissue Culture

Melaku Tesfa1*, Belayneh Admassu2 and Kassahun Bantte3
1Ethiopian Sugar Corporation Research and Training Division, Biotechnology Research Team, Wonji Research Center, Wonji, Ethiopia
2National Agricultural Biotechnology Research Program, Holetta Agricultural Research Center, Ethiopia
3Jimma University College of Agriculture and Veterinary Medicine, Jimma, Ethiopia
*Corresponding Author : Melaku Tesfa
Ethiopian Sugar Corporation Research and Training Division
Biotechnology Research Team
Wonji Research Center, Wonji, Ethiopia
Tel: 251-913241485
E-mail: [email protected]
Received: February 04, 2016; Accepted: March 17, 2016; Published: March 23, 2016
Citation: Tesfa M, Admassu B, Bantte K (2016) Ex Vitro Rooting of Sugarcane (Saccharum officinarum L.) Plantlets Derived from Tissue Culture. Adv Crop Sci Tech 4:215. doi:10.4172/2329-8863.1000215
Copyright: © 2016 Tesfa M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

A study was conducted at Holetta National Agricultural Biotechnology Laboratory with the objective to determine the effect of different concentrations of NAA on ex vitro root development of sugarcane microshoots. Five levels of NAA (0, 10, 20, 30 and 40 mg/l) and two levels of genotypes were combined in factorial arrangement. The basal end of the shoots was dipped in NAA solution overnight before the shoots were transferred into a plastic tray containing a mixed growing medium in green house. The results showed that interaction effect of genotype and NAA was highly significant (p<0.0001) on rooting percentage, number of roots per shoot, root length. In genotype N52, best root formation was found on the shoots treated with 20 mg/l NAA by which rooting percentage was 76 ± 5.48 with 5.88 ± 0.04 cm root length and 8.06 ± 0.13 number of roots per plantlets. While in genotype N53 maximum, root formation was recorded on the shoots dipped in 30 mg/l NAA by which rooting percentage was 70 ± 7.07 with 5.42 ± 0.11 cm root length and 4.52 ± 0.19 number of roots per plantlets. Shoots rooted through this method exhibited 100 % survival in both genotypes.

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