GC/MS Determination of N-butyl-N-(3-carboxypropyl) Nitrosamine (BCPN) in Bladder Cancers - The Skewed Molecular Interaction CausedRetention Time Shift
- *Corresponding Author:
- Dr. Kuan Chou Chen
Department of Urology,Taipei Medical University-Shuang Ho Hospital
Dr. Robert Y. Peng
Research Institute of Biotechnology, Hungkuang University
34 Chung-Chie Rd., Shalu County
Taichung Hsien Taiwan 43302
Tel: +886-2-27585767, +886-952-002-092
Received date: December 26, 2010; Accepted date: February 11, 2011; Published date: February 16, 2011
Citation: Hsieh CL, Wang HE, Ker YB, Peng CC, Chen KC, et al.(2011) GC/ MS Determination of N-butyl-N-(3-carboxypropyl) Nitrosamine (BCPN) in Bladder Cancers - The Skewed Molecular Interaction Caused Retention Time Shift. J Anal Bioanal Tech 1:115. doi: 10.4172/2155-9872.1000115
Copyright: © 2011 Hsieh CL, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
N-butyl-N-(4-hydroxybutyl) nitrosamine (BBN) has been widely used in rodents as an invaluable experimental tool for investigation of bladder cancer (BCA). The urinary level of its metabolite, N-butyl-N-(3-carboxypropyl) nitrosamine (BCPN) was reported to be a very reliable predicative parameter of BCA. However, in determination of the urinary BCPN we found the retention time (tR) of BCPN was randomly damping. The tR values of the authentic BCPN at 5, 10, 20, 50, and 100 ppm were 28.48, 27.59, 27.43, 28.00, and 28.32 min comparing with 28.23 min of the urinary BCPN in HPLC analysis, similarly, 17.30 min for the urinary and the 18.00 min for the authentic BCPN in GC/MS analysis. To interpret such a damping, we theoretically proposed that a certain transient skewed molecular interaction could occur during the chromatographic separation, which would cause a certain degree of fluctuation on the tR of target molecules. Conclusively, the retention time of a chemical is not a definite value as often considered in HPLC and GC/MS analyses. In reality it fluctuates depending mainly upon the interaction among a cluster of coexisting molecules, in particular, when operated at higher concentrations.