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Advances in Crop Science and Technology
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Research Article

Genetic Structure in FCV Tobacco Population as Assessed by Multi-locus Genotyping Using SSR Markers

Ganesh CT1*, Saiprasad GVS1, Mohan Raju B2, Sheshshayee MS2 and Udayakumar M2
1 Corporate R & D, ITC R&D Centre, Bangalore 560058, India
2 Department of Crop Physiology, UAS, GKVK, Bangalore 560065, Inida
Corresponding Author : Dr. Ganesh CT
Corporate R & D, ITC R&D Centre
Bangalore 560058, India
Tel: +91-9916401982
E-mail: [email protected]
Received March 28, 2014; Accepted April 25, 2014; Published April 27, 2014
Citation: Ganesh CT, Saiprasad GVS, Mohan Raju B, Sheshshayee MS and Udayakumar M (2014) Genetic Structure in FCV Tobacco Population as Assessed by Multi-locus Genotyping Using SSR Markers. Adv Crop Sci Tech 2:127. doi: 10.4172/2329-8863.1000127
Copyright: © 2014 Ganesh CT, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
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Abstract

Association mapping studies largely depend on the genetic nature of the population. An understanding of genetic structure of population assists in designing apt studies that accounts the dynamics of variability in population. Current study is aimed at analyzing genetic structure of tobacco (Nicotiana tabacum L.) population consisted of 135 FCV (Flue Cured Virginia) genotypes using multi-locus genotyping with mapped Simple Sequence Repeat (SSR) markers. 25 unlinked SSR markers delineated 135 genotypes revealing a total of 85 alleles with an average of 3.4 alleles per locus. Contrasting allelic frequencies were observed in most of the loci studied. Bayesian method of inference employed the genotyping data to derive genetic structure information from the population. High FST measures in the subpopulations (0.44, 0.09 and 0.29 at population level K=3) indicated high inbreeding coefficient signifying a strongly structured population. Evolutionary dissimilarity estimate using DARwin also supported the finding of resilient structure in the population. These findings were found highly useful in designing appropriate mapping strategies for tobacco breeding.

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