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Hydroxypropyl Methacrylate Interaction and Chitosan Coating for Enhanced UV Detection Sensitivity of Colloidal Nanoparticles in Capillary Electrophoresis Analysis | Abstract
ISSN: 2155-9872

Journal of Analytical & Bioanalytical Techniques
Open Access

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Research Article

Hydroxypropyl Methacrylate Interaction and Chitosan Coating for Enhanced UV Detection Sensitivity of Colloidal Nanoparticles in Capillary Electrophoresis Analysis

Samar Alsudir and Edward PC Lai*

Department of Chemistry, Ottawa-Carleton Chemistry Institute,Carleton University, Ottawa, ON K1S 5B6, Canada

*Corresponding Author:
Edward PC Lai
Ottawa-Carleton Chemistry Institute,Carleton University, Ottawa
ON K1S 5B6, Canada
Tel: +613-520-2600
Fax: +613-520-3749
Email: edward.lai@carleton.ca

Received date: April 08, 2015; Accepted date: April 28, 2015; Published date: May 05, 2015

Citation: Alsudir S, Lai EPC (2015) Hydroxypropyl Methacrylate Interaction and Chitosan Coating for Enhanced UV Detection Sensitivity of Colloidal Nanoparticles in Capillary Electrophoresis Analysis. J Anal Bioanal Tech 6:242. doi: 10.4172/2155-9872.1000242

Copyright: © 2015 Alsudir S, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Abstract

The binding interactions between silica (SiO2), titania (TiO2) or polymeric nanoparticles with hydroxypropyl methacrylate (HPMA) were investigated for enhancing the ultraviolet (UV) detection sensitivity of these nanoparticles in capillary electrophoresis (CE) analysis. HPMA interacted with colloidal SiO2 nanoparticles, producing a larger CE-UV peak at a slightly shorter migration time. An increase in particle size with HPMA binding was validated using dynamic light scattering. The interaction was selective as HPMA did not interact with TiO2 nanoparticles in aqueous suspension. Chitosan coating of SiO2 or TiO2 nanoparticles produced significantly larger hydrodynamic diameters to further enhance the sensitivity of their UV detection. The analytical technique, which involves coating SiO2 nanoparticles with chitosan first and binding with HPMA next, is novel. It has allowed us to achieve a significant enhancement of 50 folds in detection sensitivity.

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