Identification and Biotyping of Escherichia coli from Diarrheic Lambs in and Around Debrebirhan Town, Ethiopia
|Metasebia Aklilu1*, Tesfaye Sisay2, GeneneTefera3 and Belay Tekalign4|
|1Department of Microbiology, Immunology and Veterinary Public Health, College of Veterinary Medicine and Agriculture, Addis Ababa University, P. O. Box 34, Debrezeite, Ethiopia|
|2Department of Health Biotechnology, Institute Biotechnology, Addis Ababa University, P.O.Box 30604, Addis Ababa, Ethiopia|
|3Institute of Biodiversity Center, Addis Ababa, Ethiopia|
|4Mereced University, California, USA|
|Corresponding Author :||Metasebia Aklilu
Department of Microbiology
Immunology and Veterinary Public Health
College of Veterinary Medicine and Agriculture
Addis Ababa University
Addis Ababa, Ethiopia
E-mail: [email protected]
|Received July 20, 2013; Accepted September 25, 2013; Published October 04, 2013|
|Citation: Aklilu M, Sisay T, Tefera G, Tekalign B (2013) Identification and Biotyping of Escherichia coli from Diarrheic Lambs in and Around Debrebirhan Town, Ethiopia. J Infect Dis Ther 1:115. doi:10.4172/2332-0877.1000115|
|Copyright: © 2013 Aklilu M, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.|
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Background: Infectious diarrhea is the most significant cause of morbidity and mortality in neonatal dairy sheep throughout the world. This is the first study conducted on isolation and bio typing of E. coli from lamb diarrhea in and around DebreBirhan. The present study was undertaken with the objective of isolation, omnilog characterization and biotyping of E. coli isolates from faecal samples around Debrebrhan.
Methods: A cross-sectional study was conducted from October, 2012 to April, 2013. The study focused on lambs less than three months of age showing clinical symptoms of diarrhea. Standard cultural and Omnilog tests were done to identify E. coli species and biotypes; descriptive statistics and Chi-square were used to analyze the collected data.
Results: From a total of 100 diarrheic lambs examined, 84% were found to be positive for E. coli. On the basis of fermentation reactions of sugars, viz. dulcitol, raffinose, rhamnose, salicin, starch and sucrose, 69 isolates utilized one or more sugar, while 14 isolates failed to utilize any of the sugars. 69 E. coli isolates were bio typed into 15 different combinations. Association between different age groups and occurrence of lamb diarrhea caused by E. coli strains showed a significant association (p<0.05). From the questionnaire findings, 60% of all the health problems in lambs were due to diarrhea where young sheep were more affected.